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电刺激大鼠巨细胞网状核对小脑浦肯野细胞自发和诱发电活动的影响

张敏跃, 王建军, 王晓燕, 陈文雁, 陈均, 余启祥

南京大学生物系生理教研室. 南京 210008

摘要

该研究在麻醉并制动的大鼠上观察了电刺激巨细胞网状核(Gi)对小脑浦肯野细胞(PC)自发及诱发简单锋电位的影响。结果如下:(1)刺激Gi可使PC的简单锋电位出现潜伏期小于20ms的抑制性或兴奋性反应,并以抑制性反应为主。抑制性反应持续40-100ms,而兴奋性反应的时程可达200ms以上;(2)注射5-HT_2型受体阻断剂methysergide可以减弱或阻断电刺激Gi对PC自发简单锋电位的抑制作用;(3)条件性Gi刺激可以显著压抑或加强由刺激对侧大脑皮层感觉运动区引起的PC诱发简单锋电位反应。以上结果说明:在大鼠存在Gi-小脑通路,这一通路中的部分纤维是5-HT能的。

关键词： 巨细胞网状核; 浦肯野细胞; 简单锋电位; 巨细胞网状核-小脑投射; 5-HT能纤维; 小脑皮层

Effect of stimulation of gigantocellulars reticular nucleus on the spontaneous and evoked activities of cerebeliar purkinje cells in the rat

Zhang Minyue, Wang Jianjun, Wang Xiaoyan, Chen Wenyan, Chen Jun, Yu Qixiang

Division of Physiology, Department of Biology, Nanjing University. Nanjing 210008, China

Abstract

In anesthetized and paralyzed rats,the effect of stimulation of Gigantocellulars
Reticular Nucleus (Gi) on spontaneous and evoked simple spike discharges of cerebellar Purkinje cells (PCs) was examined. The result were as follows:(1) Stimulation of Gi elicited either inhibitory or excitatory effect on spontaneous simple spikes of PCs with latency less than 20 ms，being inhibitory in the most cases. The inhibitory responses lasted for 40-100 ms，while the excitatory ones were sometimes longer than 200 ms;(2)Injection of 5-HT_(2) recptor antagonist methysergide could reversibly attenuate or block the inhibitory effect of Gi stimulation on spontaneous simple spike discharges of PCs;(3) The evoked simple spike responses of PCs elicited by stimulating the contralateral cerebral sensorimotor cortex could be markedly suppressed or otentiated by the conditioning Gi stimulation.The results demonstrated that Gi-cerebellar pathways exists，with some fibers being serotonergic. Gi-cerebellar afferent fibers may have certain modulatory effects. on PCs through synaptic and/or non-classical chemical synaptic transmission. The Gicerebellar afferent fiber projection may play important roles in some cerebellar functions，such as regulating myotonia and posture.

Key words: Gigantocellulars Reticular Nucleus;Cerebellar cortex;Purkinje cell;Simple spike;Giantocellulars-cerebellar projection;Serotonergic fiber

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引用本文：

张敏跃, 王建军, 王晓燕, 陈文雁, 陈均, 余启祥. 电刺激大鼠巨细胞网状核对小脑浦肯野细胞自发和诱发电活动的影响[J]. 生理学报 1993; 45 (1): .

Zhang Minyue, Wang Jianjun, Wang Xiaoyan, Chen Wenyan, Chen Jun, Yu Qixiang. Effect of stimulation of gigantocellulars reticular nucleus on the spontaneous and evoked activities of cerebeliar purkinje cells in the rat. Acta Physiol Sin 1993; 45 (1): (in Chinese with English abstract).