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丹参酮Ⅱ-A磺酸钠对分离的豚鼠心室肌单细胞慢反应动作电位的作用

刘启营, 蔡体导

中国科学院上海生理研究所

摘要

分离的成年豚鼠心室肌单细胞在高钾(25mmol/L)溶液中部分除极化使钠通道失活,用细胞内刺激诱发慢反应动作电位。20μmol/L 的丹参酮Ⅱ-A 磺酸钠对这种慢反应动作电位有明显的抑制作用。在1—20μmol/L 浓度范围内,丹参酮Ⅱ-A 磺酸钠对0.28μmol/L 的异丙肾上腺素强化的慢反应动作电位有浓度依赖性抑制作用。而且,随异丙肾上腺素浓度的增加(69 nmol/L-0.55μmol/L)抑制作用更加明显。以上结果提示丹参酮 Ⅱ-A 磺酸钠可能是一种钙拮抗剂。50-100μmol/L 的丹参酮 Ⅱ-A 磺酸钠使分离的成年豚鼠心室肌单细胞快反应动作电位的幅度降低,达峰值的时间延长。

关键词： 分离的心室肌细胞; 慢反应动作电位; 丹参酮 Ⅱ-A 磺酸钠

Effects of sodium tanshinone Ⅱ-A sulfonate on slow action potentials of dissociated single ventrlcular cells of adult guinea pig heart

Liu Qiying, Tsai Tidao

Shanghai Institute of Physiology, Chinese Academy of Science

Abstract

The sodium channels of dissociated single ventricular cells of adult guinea
pig heart were inactivated by partial depolarization in high K~(+) (25 mmol/L)
Tyrode's solution and slow response action potential was elicited by intracellular stimulation. An obvious inhibition of the response was observed in the presence of 20#mu#mol/L sodium tanshinone Ⅱ-A sulfonate(DS-201). In the concentration range from 1#mu#mol/L to 20#mu#mol/L, the inhibition effect of sodium tanshinone Ⅱ-A sulfonate on the slow response action potentials enhanced by 0.28#mu#mol/L isoprenaline is concentration-dependent. Moreover, the inhibition effects of sodium tanshinone Ⅱ-A sulfonate become stronger with the increase (In the range of 6.9 nmol/L to 0.55#mu#mol/L) of isoprenaline. The abovementioned results suggest that sodium tanshinone Ⅱ-A sulfonate may be a kind of effective calcium channel blocker.

Key words: Dissociated ventricular myocytes;Slow response action potential;Sodium tanshinone Ⅱ-A sulfonate

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引用本文：

刘启营, 蔡体导. 丹参酮Ⅱ-A磺酸钠对分离的豚鼠心室肌单细胞慢反应动作电位的作用[J]. 生理学报 1990; 42 (3): .

Liu Qiying, Tsai Tidao. Effects of sodium tanshinone Ⅱ-A sulfonate on slow action potentials of dissociated single ventrlcular cells of adult guinea pig heart. Acta Physiol Sin 1990; 42 (3): (in Chinese with English abstract).