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八肽胆囊收缩素对抗mu和kappa型受体介导的镇痛作用

王霄虹, 王晓京, 韩济生

北京医科大学. 北京；北京医科大学生理教研室. 北京

摘要

以往的资料表明,八肽胆襄收缩素(CCK-8)能对抗阿片镇痛,本实验进一步分析 CCK-8对抗哪一类型阿片受体激动剂的镇痛作用。给大鼠脊髓蛛网膜下腔(I.T.)注射 CCK-8(剂量4ng到1.0μg)既不产生痛敏也不产生镇痛。I.T.注射特异性的μ受体激动剂 PL01710 ng 或 k 受体激动剂 NDA P500 ng 引起的镇痛作用可被注射 CCK-8 4ng 所对抗。而L.T.注射δ受体激动剂 DPDPE(6.5,13.0和26.Oμg)引起的镇痛作用不能被 CCK-8(4ng,40ng I.T.)所对抗。但 CCK-8对抗 PL017和 NDAP 镇痛的作用可被 I.T.CCK 受体拮抗剂 proglumide(3μg)所翻转。

关键词： CCK-8; PL017; NDAP; Proglumide; 鞘内注射

Cholecystokinin octapeptide (CCK-8) antagonized nalgesia mediated by mu and kappa opioid receptors

Wang Xiaohong, Wang Xiaojing, Han Jisheng

Department of Physiology, Beijing Medical University. Beijing, China

Abstract

CCK-8 has been shown to antagonize the analgesia produced by morphine or
endogenous opioid peptides. The present study was performed to clarify the interaction between CCK-8 and different opioid ligands. Analgesia produced by
intrathecal (I.T.) injection of the specific #mu# receptor agonist PL017 10ng or #kappa# receptor agonist NDAP 500 ng can be antagonized by I.T. injection CCK-8 at a dose as small as 4 ng. In contrast, analgesia produced by I.T. injection of the #delta# receptor agonist DPDPE (6.5,13 and 26 #mu#g) was not blocked by CCK-8 (4 ng or 40 ng, I.T.). The antagonistic effect of CCK-8 on PL017 and NDAP could be completely reversed by progiumide (3#mu#g, I.T.).I.T. injection of CCK-8 (4 or 40 ng single dose or cummulative dose of 0.1,0.2,0.5 and 1.0#mu#g at 10 min intervals) produced neither analgesia nor hyperalgesia. In conclusion, CCK-8 preferentially antagonizes opioid analgesia mediated by #mu# and #kappa# receptors, and this antagonistic effect is mediated by CCK receptors.

Key words: CCK-8;PL017;NDAP;Proglumide;Intrathecal injection

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引用本文：

王霄虹, 王晓京, 韩济生. 八肽胆囊收缩素对抗mu和kappa型受体介导的镇痛作用[J]. 生理学报 1990; 42 (3): .

Wang Xiaohong, Wang Xiaojing, Han Jisheng. Cholecystokinin octapeptide (CCK-8) antagonized nalgesia mediated by mu and kappa opioid receptors. Acta Physiol Sin 1990; 42 (3): (in Chinese with English abstract).