过刊浏览

可视膜片钳法记录初级传入纤维介导的脊髓背角神经元突触后电流

万业宏, 王玉英, 戴飞, 胡三觉

第四军医大学基础部神经科学研究所. 西安 710033

摘要

本文描述了用明胶半包埋法制备带背根脊髓薄片的实验步骤,和在脊髓背角记录由初级传入纤维介导的突触后电流的可视膜片钳法.手术制备一段带背根的脊髓标本,并用20%的明胶包埋在琼脂块上,再用振动切片机切片获得带背根的脊髓薄片.通过红外线可视的引导,在脊髓背角神经元上建立全细胞封接模式.在钳制电压为-70 mV条件下,记录自发的和背根刺激引起的兴奋性突触后电流。

关键词： 膜片钳技术; 兴奋性突触后电位; 抑制性突触后电位; 初级传入突触; 脊髓; 背角; 大鼠

Visually guided patch-clamp recording of spinal dorsal horn neuron's postsynaptic current evoked by primary afferent fiber

Wan Yehong, Wang Yuying, Dai Fei, Hu Sanjue

Institute of Neuroscience, The Fourth Military Medical University. Xi'an. Shaanxi 710033, China

Abstract

The authors describe here the procedures for using the gelatin half-embedding method to obtain thin spinal cord slices with attached dorsal roots and performing visually guided whole-cell patch-clamp recording of postsynaptic currents evoked by primary afferent fibers in rat spinal dorsal horn. A segment of spinal cord with attached dorsal roots was prepared and half-embedded in an agar block with 20% (w/v)gelatin. Thin spinal cord slices with attached dorsal roots were obtained with a vibratome and whole-cell patch-clamp configuration was established under the infrared observation. At the holding potential of -70 mV, spontaneous excitatory postsynaptic currents (EPSCs) and dorsal root stimulation-evoked EPSCs were recorded as inward currents.

Key words: patch-clamp technique;Excitatory postsynaptic current (EPSC);Inhibitory postsynaptic currents (IPSC);Primaryafferent synapse;Spine;Dorsal horn;Rat

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

万业宏, 王玉英, 戴飞, 胡三觉. 可视膜片钳法记录初级传入纤维介导的脊髓背角神经元突触后电流[J]. 生理学报 2004; 56 (4): .

Wan Yehong, Wang Yuying, Dai Fei, Hu Sanjue. Visually guided patch-clamp recording of spinal dorsal horn neuron's postsynaptic current evoked by primary afferent fiber. Acta Physiol Sin 2004; 56 (4): (in Chinese with English abstract).