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诺沙坦改善非胰岛素依赖型糖尿病大鼠胰岛素敏感性的作用机制

吴勇, 欧阳静萍, 周云峰, 吴柯, 赵德海, 文重远

武汉大学医学院病理生理教研室,武汉,430071；湖北中医学院生理教研室,武汉,430061

摘要

本文研究血管紧张素Ⅱ受体拮抗剂诺沙坦对非胰岛素依赖型糖尿病(non-insulin-dependent diabetes mellitus,NIDDM)大鼠胰岛素敏感性的改善作用,并探讨其作用机制.从饮水中给予正常或高脂喂养加小剂量链脲佐菌素(STZ)诱发的NIDDM大鼠诺沙坦(4 mg/kg),连续6周。

关键词： 诺沙坦; 胰岛素敏感性; 非胰岛素依赖型糖尿病 (NIDDM); GLUT4转位; 链脲佐菌素

Mechanism of improving effect of losartan on insulin sensitivity of non-insulin-dependent diabetes mellitus rats

Ding Juan

Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan. Hubei 430022, China

Abstract

The specific inhibition of angiotensin Ⅱ action at AT1 receptors by losartan has been shown to decrease peripheral insulin resistance in type 2 diabetic patients and animal models. We examined the effect of losartan on the expression of insulin receptor substrate 1 (IRS-1), protein kinase B (PKB) and glucose transporter 4 (GLUT4), as well as the phosphorylation status of IRS-1 and the association between IRS-1 and phosphatidylinositol (PI) 3-kinase in skeletal muscle from fat-fed and-streptozotocin (STZ)-treated rats, an animal model of type 2 diabetes mellitus. In addition, the effects of losartan on GLUT4 translocation in muscle cells and on insulin sensitivity were also evaluated. Muscle tissues were isolated from male losartan-treated and untreated normal or non-insulin-dependent diabetes mellitus (NIDDM) rats with a dose of 4 mg/kg per day for 6 weeks.

Key words: Losartan;Insulin sensitivity;Non-insulin-dependent diabetes mellitus (NIDDM);GLUT4 translocation;

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引用本文：

吴勇, 欧阳静萍, 周云峰, 吴柯, 赵德海, 文重远. 诺沙坦改善非胰岛素依赖型糖尿病大鼠胰岛素敏感性的作用机制[J]. 生理学报 2004; 56 (4): .

Ding Juan. Mechanism of improving effect of losartan on insulin sensitivity of non-insulin-dependent diabetes mellitus rats. Acta Physiol Sin 2004; 56 (4): (in Chinese with English abstract).