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利诺吡啶对豚鼠耳蜗外毛细胞和Deiters细胞钾电流的抑制

龚树生, 常青, 丁娟

华中科技大学同济医学院附属协和医院耳鼻咽喉科. 湖北, 武汉 430022

摘要

为探讨KCNQ家族钾通道在耳蜗外毛细胞和Deiters细胞的功能性表达,我们观察并记录了KCNQ家族钾通道阻滞剂利诺吡啶对豚鼠耳蜗单离外毛细胞(outer hair cells,OHCs)和Deiters细胞总钾电流的影响.采用酶孵育加机械分离法分离豚鼠耳蜗单个OHCs和Deiters细胞;运用膜片钳技术,在全细胞模式下记录正常细胞外液中8个外毛细胞和5个Deiters细胞的总钾电流,并观察100 μmol/L和200 μmol/L利诺吡啶对外毛细胞和Deiters细胞总钾电流的影响。

关键词： KCNQ; 钾通道; 外毛细胞; Deiters 细胞; 膜片钳技术; 利诺吡

Inhibition of potassium currents in outer hair cells and Deiters' cells from guinea pig cochlea by linopirdine

Gong Shusheng, Chang Qing, Ding Juan

Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan. Hubei 430022, China

Abstract

To study the functional expression of KCNQ gene in outer hair ceils (OHCs) and Deiters' ceils, the effects of linopirdine on the whole cell K+ current were investigated by using the whole cell variant of patch clamp technique in the present study. The outward tetraethylammonium (TEA)-sensitive K+ current and the inward K+ current (IKn) in OHCs were recorded and measured before and after the administration of linopirdine. Simultaneously, the whole cell currents in Deiters' cells were also observed in normal solution and in the presence of linopirdine. After the application of 100 μmol/L linopirdine to OHCs, the peak K+ current was reversibly blocked and the late K+ current was partly reduced. In addition, the decay time constant of the TEA-sensitive K+ current was prolonged in the presence of 100 μmol/L linopirdine. The inward current in OHCs was totally inhibited after the superfusion of 100 μmol/L and 200 μrnol/L linopirdine respectively.

Key words: KCNQ;Potassium channels;Outer hair cells;Deiters cells;patch clamp technique;Linopirdine

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引用本文：

龚树生, 常青, 丁娟. 利诺吡啶对豚鼠耳蜗外毛细胞和Deiters细胞钾电流的抑制[J]. 生理学报 2004; 56 (4): .

Gong Shusheng, Chang Qing, Ding Juan. Inhibition of potassium currents in outer hair cells and Deiters' cells from guinea pig cochlea by linopirdine. Acta Physiol Sin 2004; 56 (4): (in Chinese with English abstract).