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迁移的鼻咽癌细胞容积激活性氯电流

毛建文, 王立伟, 孙雪荣, 朱林燕, 李攀, 钟平, 聂思槐, Tim JACOB, 陈丽新

广东医学院细胞生物学研究室,湛江 524023；广东医学院生理研究室,湛江 524023；School of Biosciences,cardiff CF10 3US.UK

摘要

用膜片钳技术研究了Transwell小室趋化迁移后的鼻咽癌CNE-2Z细胞容积激活性Cl-电流.47%低渗刺激迁移后的CNE-2Z细胞诱发容积激活性氯电流,与未迁移细胞相比,其特性以及其对氯通道阻断剂的敏感性发生明显的变化,此电流的密度明显高于未迁移细胞,而且该电流几乎完全被氯通道阻断剂adenosine-5'-triphosphate(ATP,10 mmol/L)、5-nitro-2-3-phenylpropylamino benzoic acid(NPPB,100μmol/L)和他莫昔芬(30 μmol/L)抑制,其中NPPB和他莫昔芬对迁移细胞的抑制作用明显强于未迁移细胞.迁移后的CNE-2Z细胞容积激活性氯通道对阴离子的通透性为:Br-＞Cl-＞Y＞葡萄糖酸,与未迁移细胞(I＞Br-＞Cl-＞葡萄糖酸)不同.结果提示,容积激活性氯通道可能参与CNE-2Z细胞的迁移过程。

关键词： 肿瘤细胞; 培养的; 氯通道; 细胞迁移; 膜片钳技术

Volume-activated Cl- current in migrated nasopharyngeal carcinoma cells

Qian Lingjia

Institute of Hygiene and environmental Medicine, Academy of Military Medical Sciences. Tianjin 300050, China

Abstract

The transwell chamber migration assay and the patch-clamp technique were used to investigate the volume-activated Cl- current (ICl.vol) in migrated nasopharyngeal carcinoma cells (CNE-2Z). 47％ hypotonic solution activated a ICl.vol in the migrated CNE-2Z cells.Compared with the control cells (non-migrated), the properties of this current and the sensitivity to Cl- channel blockers were changed.The current density in migrated CNE-2Z cells was higher than that in non-migrated cells. The current was almost completely inhibited by extracellular application of adenosine-5'-triphosphate (ATP, 10 mmol/L), 5-nitro-2-3-phenylpropylamino benzoic acid (NPPB, 100mmol/L) and tamoxifen (30 mmol/L) in all voltage steps applied. The inhibition of NPPB and tamoxifen on the current was stronger in migrated cells than that in non-migrated cells. The permeability sequence of the four anions was Br-＞Cl-＞I-＞Gluconate. The sequence was different from that of the non-migrated cells (I-＞ Br-＞Cl-＞Gluconate). The results suggest that volume-activated chloride channels may be involved in the CNE-2Z cell migration.

Key words: Tumor cells;Cultured;Chloride channels;Cell migration;Patch-clamp techniques

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引用本文：

毛建文, 王立伟, 孙雪荣, 朱林燕, 李攀, 钟平, 聂思槐, Tim JACOB, 陈丽新. 迁移的鼻咽癌细胞容积激活性氯电流[J]. 生理学报 2004; 56 (4): .

Qian Lingjia. Volume-activated Cl- current in migrated nasopharyngeal carcinoma cells. Acta Physiol Sin 2004; 56 (4): (in Chinese with English abstract).