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应激对同型半胱氨酸代谢的负性调节

吴淑庆, 钱令嘉

军事医学科学院卫生学环境医学研究所. 天津 300050

摘要

基于应激对高同型半胱氨酸血症具有诱导作用,本文探索了应激致同型半胱氨酸(homocysteine,HCY)代谢变化的环节,并初步揭示了该作用的意义.以束缚应激法建立大鼠应激模型,采用高压液相-荧光检测法测定血浆HCY水平,用放射性酶学法检测不同组织中胱硫醚β合成酶(cystathionine beta-synthase,CBS)活性的变化,以及RT-PCR法和Northern blot法检测CBS mRNA水平的变化。

关键词： 代谢; 应激; 同型半胱氨酸; 胱硫醚β合成酶

Negative regulation of homocysteine metabolism by stress in rats

Wu Shuqing, Qian Lingjia

Institute of Hygiene and environmental Medicine, Academy of Military Medical Sciences. Tianjin 300050, China

Abstract

To investigate the effect of stress on homocysteine metabolism in the rat and explore the mechanism as well as the key regulatory link of stress-induced hyperhomocysteinemia, male Wistar rats were treated with restraint stress while control rats received routine treatment. By HPLC-fluorometry, the homocysteine level in rat plasma was determined. Cystathionine beta-synthase (CBS) activity in blood, heart, liver and kidney was measured by radioisotope assay using [14C]-serine as the labeled substrate. Total RNA was isolated from rat liver after restraint stress. RT-PCR and Northern blot were used to estimate the level of CBS mRNA. The results showed that hyperhomocysteinemia was induced by restraint stress. The highest CBS enzyme activity was seen in rat livers. A decrease in hepatic activities of CBS was found in restraint stress rats. The 29.4%±2.5% reduction in the activity of CBS was accompanied by a 44.1% ± 3.4% decrease in its mRNA level.

Key words: Metabolism;Stress;Homocysteine;Cystathionine beta-synthase

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引用本文：

吴淑庆, 钱令嘉. 应激对同型半胱氨酸代谢的负性调节[J]. 生理学报 2004; 56 (4): .

Wu Shuqing, Qian Lingjia. Negative regulation of homocysteine metabolism by stress in rats. Acta Physiol Sin 2004; 56 (4): (in Chinese with English abstract).