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粘着斑激酶在bFGF引起细胞迁移中的动态变化及意义

梁光波, 张国平, 金惠铭, 钱睿哲

复旦大学上海医学院生理与病理生理学系. 上海 200032

摘要

本文旨在观察不同浓度碱性成纤维细胞生长因子(basicfibroblast growthfactor,bFGF)引起体外培养的ECV-304细胞迁移时粘着斑激酶(focal adhesion kinase,FAK)的动态变化及FAK与细胞迁移的关系.建立体外培养的ECV-304细胞划痕损伤模型,观察经不同剂量(0、5、10、15 ng/ml)bFGF作用12～24 h内细胞迁移距离(电脑图像测定)和FAK蛋白含量(Western blot)、活性(免疫沉淀加Western blot)和mRNA(RT-PCR)的动态变化。

关键词： 迁移; 粘着斑激酶(FAK); 碱性成纤维细胞生长因子(bFGF); 整合素α3

Dynamic changes in focal adhesion kinase during cell migration induced bybFGF and the significance

Liang Guangbo, Zhang Guoping, Jin Huiming, Qian Ruizhe

Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University. Shanghai 200032, China

Abstract

Cell migration plays an important role in repair of injury, angiogenesis, cancer metastasis and so on. In this paper the effects of basic fibroblast growth factor (bFGF) of different concentrations on ECV-304 cell migration, and the dynamic changes in focal adhesion kinase (FAK) were observed. The relationship between FAK and cell migration induced by bFGF was studied. A ECV-304 cell scratch wound model was established and the images of cell migration were quantitatively measured using a computer-assisted videomicroscopic system.

Key words: Migration;FAK;bFGF;integrin #alpha#3

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引用本文：

梁光波, 张国平, 金惠铭, 钱睿哲. 粘着斑激酶在bFGF引起细胞迁移中的动态变化及意义[J]. 生理学报 2004; 56 (4): .

Liang Guangbo, Zhang Guoping, Jin Huiming, Qian Ruizhe. Dynamic changes in focal adhesion kinase during cell migration induced bybFGF and the significance. Acta Physiol Sin 2004; 56 (4): (in Chinese with English abstract).