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胍丁胺对大鼠穹隆下器神经元电活动的影响

王泽民, 季淑梅, 张浩, 孙光启, 王志安, 何瑞荣

河北医科大学基础医学研究所生理室. 河北, 石家庄 050017

摘要

应用细胞外记录单位放电技术,在73个大鼠穹隆下器脑片上观察了胍丁胺(agmatine,Agrm)对神经元电活动的影响.实验结果如下:(1)在28个穹隆下器脑片上灌流Agm(1.0 μmol/L)2 min,有24个单位(85.7%)自发放电频率明显降低,4个单位(14.3%)无明显变化;(2)预先用L-谷氨酸(0.3 mmol/L)灌流,24个放电单位中有19个单位(79.2%)放电频率明显增加,表现为癫痫样放电,5个单位(20.8%)的变化不明显,在此基础上灌流Agm(1.0 μmol/L)2 min,有15个单位(78.9%)的癫痫样放电被抑制,另外4个单位(21.1%)无明显变化。

关键词： 穹隆下器; 胍丁胺; L-谷氨酸; Bay K-8644; N-硝基-L-精氨酸甲酯

Effects of agmatine on the electrical activity of subfomical organ neurons

Wang Zemin, Ji Shumei, Zhang Hao, Sun Guangqi, Wang Zhian, He Ruirong

Department of Physiology, Institute of Basic Medicine, Hebei Medical University. Shijiazhuang 050017, China

Abstract

The aim of this study was to investigate the effects of agmatine (Agm) on the electrical activity of neurons in subfornical organ (SFO) slices using extracellular recording technique. The results are as follows. (1) In response to the application of Agm (1.0 #mu#mol/L) into the superfusate for 2 min, the discharge rate of 24/28 (85.7%) subfomical neurons was decreased significantly, while the discharge rate of 4/28 (14.3%) neurons were not affected. (2) Pretreatment with L-glutamate (0.3 mmol/L) led to a marked increase in the discharge rate of 19/24 (79.2%)subfomical neurons in an epileptiform pattern and the activity of the remaining 5/24 (20.8%) neurons was unaffected. By application of Agm (1.0 #mu#mol/L) into the superfusate for 2 min, the epileptiform dicharge of 15/19 (78.9%) neurons was suppressed significantly, while that of the other 4 (21.1%) neurons was not inhibited.

Key words: Subfornical organ;Agmatine;L-glutamate;Bay K-8644;NG-nitro-L-arginine methyl ester

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引用本文：

王泽民, 季淑梅, 张浩, 孙光启, 王志安, 何瑞荣. 胍丁胺对大鼠穹隆下器神经元电活动的影响[J]. 生理学报 2004; 56 (4): .

Wang Zemin, Ji Shumei, Zhang Hao, Sun Guangqi, Wang Zhian, He Ruirong. Effects of agmatine on the electrical activity of subfomical organ neurons. Acta Physiol Sin 2004; 56 (4): (in Chinese with English abstract).