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三羟异黄酮对豚鼠心室肌细胞L-型钙通道电流的影响

吉恩生, 尹京湘, 马会杰, 何瑞荣

河北医科大学基础医学研究所生理室,石家庄,050017

摘要

本实验用全细胞膜片钳技术观察三羟异黄酮(genistein,GST)对豚鼠心室肌细胞L-钙通道电流(Ica,L)的影响.结果如下:(1)GST(10、50、100μmol/L)可浓度依赖性地降低Ica,L(n=6,P＜0.01).GST的非活性结构类似物daidzein(100 μmol/L),在同一浓度范围对ICa,L没有影响(n=5,P＞0.05).(2)GST使I-V曲线上移,但对ICa,L的电压依赖特征和最大激活电压无明显影响。

关键词： 三羟异黄酮; 膜片箝技术; 心肌; L-型钙通道

Effect of genistein on L-type calcium current in guinea pig ventricular myocytes

Han Taizhen

Department of Physiology, School of Medicine, Xi'an Jiaotong University. Xi'an 710061, Shanxi, China

Abstract

This paper was aimed to study the effect of genistein (GST) on L-type calcium current (ICa,L)in isolated guinea pig ventricular myocytes using whole cell patch-clamp recording technique. The results are as follows. (1) GST (10, 50, 100 μmol/L) reduced the voltageactivated peak amplitude of ICa,L in a concentration-dependent manner. Daidzein (100 μrnol/L), a structural analogue of GST which has little or no inhibitory effect on tyrosine kinase, produced no effect over the same concentration range on ICa,L (n=5, P＞0.05). (2) GST up- shifted the current-voltage (I-V) curve, but the characteristics of I-V relationship were not significantly altered, and the maximal activation voltage of ICa, L was not different from that of control. GST did not affect the activation kinetics of ICa, L.

Key words: Genistein;Patch-clamp techniques;Myocardium;L-type calcium channels

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引用本文：

吉恩生, 尹京湘, 马会杰, 何瑞荣. 三羟异黄酮对豚鼠心室肌细胞L-型钙通道电流的影响[J]. 生理学报 2004; 56 (4): .

Han Taizhen. Effect of genistein on L-type calcium current in guinea pig ventricular myocytes. Acta Physiol Sin 2004; 56 (4): (in Chinese with English abstract).