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一种新的体外细胞牵张刺激装置

席雨涛, 白晓君, 吴格如, 马爱群

西安交通大学第一医院心内科, 教育部环境与疾病相关基因重点实验室. 西安 710061

摘要

体外细胞机械刺激装置是研究细胞对机械牵张反应的一种研究设备.目前此类装置有多种,但是这些装置都存在一些不便之处.本文以商品化的Flexercell Strain Unit刺激装置为参照,设计一套离心力牵张装置.通过使贴壁细胞生长的培养板以一定的速度旋转,从而使心肌细胞受到固定大小的离心力的牵张作用.酶解分离3～5 d SD大鼠心肌细胞,并分别给予12和24 h机械刺激:传统的20%牵张刺激和180 r/min的离心力刺激.以3H-亮氨酸掺入量反映细胞的肥大指标,并测定牵张引起的血管紧张素Ⅱ的自分泌。

关键词： 牵张; 心肌细胞; 肥大; 技术

Centrifugal force stretcher a new of in vitro mechanical cell stimulator

Xi Yutao, Bai Xiaojun, Wu Geru, Ma Aiqun

Department of Cardiology, the First Hospital, Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education, Xi'an Jiaotong University. Xi'an 710061, China

Abstract

A number of mechanical cell stimulators have been used to study the effect of mechanical stimulation on cells in vitro. But the efficiency of these devices is not fully desirable. We recently developed a new device for mechanical cell stimulation, the centrifugal force stretcher, and compared its efficacy with that of the traditional Flexercell Strain Unit. When the mechanical stretcher circumrotates with certain speed, cardiac myocytes attached on the plate are stretched and elongated by centrifugal force. Neonatal rat cardiac myocytes were isolated by enzymatic dissociation from the hearts of 3～5 d old Sprague Dawley rats, and were mechanically stimulated by traditional 20%stretch and 180 r/min centrifugal force for 12 and 24 h. The effects of mechanical stimulation on the hypertrophic response of neonatal rat cardiac myocytes and production of angiotensin Ⅱ (Ang Ⅱ) were examined.

Key words: Sstretcher;Cardiac myocytes;Hypertrophy;Technique

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引用本文：

席雨涛, 白晓君, 吴格如, 马爱群. 一种新的体外细胞牵张刺激装置[J]. 生理学报 2004; 56 (3): .

Xi Yutao, Bai Xiaojun, Wu Geru, Ma Aiqun. Centrifugal force stretcher a new of in vitro mechanical cell stimulator. Acta Physiol Sin 2004; 56 (3): (in Chinese with English abstract).