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依托咪酯对成年大鼠脊髓胶状质局部突触传递的作用

李震, 罗层, 孙焱芫, 陈军

第四军医大学全军神经科学研究所. 西安 710032

摘要

应用盲插全细胞膜片钳技术,在成年大鼠脊髓薄片上观察依托咪酯(etomidate,ET)对脊髓胶状质局部突触传递的影响.实验结果显示,在钳制电压为-70 mV时,500μmol/L的ET对微小兴奋性突触后电流(mEPSC)的持续时间、频率和幅度都无明显的作用。

关键词： 依托咪酯; 脊髓; 胶状质; 突触传递

Effects of etomidate on local synaptic transmission in substantia gelatinosa neurons of the adult rat spinal cord

Li Zhen, Luo Ceng, Sun Yanyan, Chen Jun

Institute of Neuroscience, Fourth Military Medical University. Xi'an 710032, China

Abstract

By using blind spinal slice whole-cell patch-clamp technique, we observed the influence of etomidate (ET) on synaptic transmission in substantia gelatinosa neurous of the adult rat spinal core. Male sdult Sprague-Dawley rats (7-8 weejs old) were anaesthetized with urethane (1.2g/kg. i. p.), and the lumbosacral laminectomy was performed. The Lumbosacral spinal cord (L1-S3) was removed and placed in preoxygenated Krebs solution at 1-3℃.

Key words: Etomidate;Spinal cord;Substantia gelatinosa;Synaptic transmission

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引用本文：

李震, 罗层, 孙焱芫, 陈军. 依托咪酯对成年大鼠脊髓胶状质局部突触传递的作用[J]. 生理学报 2004; 56 (3): .

Li Zhen, Luo Ceng, Sun Yanyan, Chen Jun. Effects of etomidate on local synaptic transmission in substantia gelatinosa neurons of the adult rat spinal cord. Acta Physiol Sin 2004; 56 (3): (in Chinese with English abstract).