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肢体缺血预处理减少大鼠全脑缺血再灌注诱导的海马CA1区锥体神经元凋亡

赵红岗, 李文斌, 李清君, 陈晓玲, 刘慧卿, 冯荣芳, 艾杰

河北医科大学基础医学研究所病理生理学研究室. 河北, 石家庄 050017

摘要

探讨肢体缺血预处理(limb ischemic preconditioning,LIP)对大鼠全脑缺血再灌注后海马CA1区锥体细胞凋亡的影响.46只大鼠椎动脉凝闭后分为假手术组、肢体缺血组、脑缺血组、LIP组.重复夹闭大鼠双侧股动脉3次(每次10 min,间隔10 min)作为LIP,之后立即夹闭双侧颈总动脉进行全脑缺血8 min后再灌注.DNA凝胶电泳、TUNEL和吖啶橙/溴乙锭(AO/EB)双染技术从生化和形态学方面观察海马神经元凋亡的情况.凝胶电泳显示,脑缺血组出现了凋亡特征性DNA梯状条带,而LIP组无上述条带出现。

关键词： 病理生理学; 肢体缺血预处理; 脑缺血; 凋亡; 海马; 大鼠

Limb ischemic preconditioning attenuates apoptosis of pyramidal neurons in the CA1 hippocampus induced by cerebral ischemia-reperfusion in rats

Zhao Honggang, Li Wenbin, Li Qingjun, Chen Xiaoling, Liu Huiqing, Feng Rongfang, Ai Jie

Department of Pathophysiology, Institute of Basic Medicine, Hebei Medical University. Shijiazhuang 050017, China

Abstract

The purpose of this study was to investigate the effects of limb ischemic preconditioning (LIP) on apoptosis of pyramidal neurons in the CA 1 hippocampus induced by global cerebral ischemia-reperfusion in rats. Forty-six rats whose bilateral vertebral arteries were occluded permanently were assigned to one of four groups: sham group, limb ischemia group, cerebral ischemia group and LIP group. LIP was performed by occluding the bilateral femoral arteries for 10 min 3 times in an interval of 10 min. Global cerebral ischemia was underwent by occluding the bilateral common carotid arteries for 8 min immediately after LIP. Assays for apoptosis of the hippocampal neurons were biologically and morphologically performed using gel electrophoresis, TUNEL and AO/EB staining. Characteristic DNA ladder was clearly visualized with gel electrophoresis in the hippocampus in cerebral ischemia group, but not in LIP group.

Key words: Pathophysiology;Limb ischemic preconditioning;Cerebral ischemia;Apoptosis;Hippocampus;Rat

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引用本文：

赵红岗, 李文斌, 李清君, 陈晓玲, 刘慧卿, 冯荣芳, 艾杰. 肢体缺血预处理减少大鼠全脑缺血再灌注诱导的海马CA1区锥体神经元凋亡[J]. 生理学报 2004; 56 (3): .

Zhao Honggang, Li Wenbin, Li Qingjun, Chen Xiaoling, Liu Huiqing, Feng Rongfang, Ai Jie. Limb ischemic preconditioning attenuates apoptosis of pyramidal neurons in the CA1 hippocampus induced by cerebral ischemia-reperfusion in rats. Acta Physiol Sin 2004; 56 (3): (in Chinese with English abstract).