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脊髓背角Ⅱ板层长时程增强诱导及维持过程中的突触形态计量学研究

项旭映, 张红梅, 胡能伟, 周利君, 张彤, 刘先国

中山大学中山医学院生理学教研室. 广东, 广州 510089

摘要

本研究用体视学方法探讨了在C纤维诱发电位长时程增强(1ong-term potentiation,LTP)的诱导及维持过程中的脊髓背角Ⅱ板层的突触形态变化.结果显示:(1)在LTP形成后30 min,Ⅱ板层内的突触后致密物质(postsynaptic density,PSD)增厚,突触间隙增宽;(2)在LTP形成后3 h,PSD厚度、突触间隙宽度及突触界面曲率都有明显增加;(3)在LTP诱导和维持全过程中,总突触的数密度比对照组有明显增高.(4)在LTP形成后3 h和5 h,穿孔性突触的数密度与对照组比较有明显增高.上述结果显示:PSD增厚是LTP诱导阶段的主要形态学变化.突触界面曲率增大及穿孔突触数目增多是LTP维持阶段的主要形态学基础。

关键词： 长时程增强; 脊髓背角; 突触

A quantitative study of the synaptic alterations in spinal dorsal horn during the induction and maintenance of long-term potentiation

Xiang Xuying, Zhang Hongmei, Hu Nengwei, Zhou Lijun, Zhang Tong, Liu Xianguo

Department of Physiology, Zhongshan Medical School of Sun Yat-Sen University. Guangzhou 510089, China

Abstract

By using stereological morphometric techniques, we examined the ultrastructure of synapses in lamine Ⅱ of the spinal dorsal horn of Sprague Dawley rats 30 min, 3 h and 5 h after long-term potentiation (LTP) induction. We found that the numerical density per unit volume (Nv) of total synapses, the thickness of the postsynaptic density (PSD), width of the synaptic cleft increased significantly after the establishment of LTP. (1) Thirty minutes after the formation of LTP, the thickness of the PSD increased from 0.029±0.0064μm (control) to 0.036±0.009μm (P<0.05) and the width of the synaptic cleft increased from 0.0181±0.0024 μm (control) to 0.0197±0.0029μm (P<0.05); the number of synaptic vesicles decreased from 0.122±0.0l1/μm~2 to 0.085±0.010μm~2 (P<0.05); (2) 3 h after the formation of LTP, the thickness of PSD and the width of the synaptic cleft had no difference compared with those 30 min after LTP.

Key words: Long-term potentiation （LTP）;Spinal dorsal horn;Synapse

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引用本文：

项旭映, 张红梅, 胡能伟, 周利君, 张彤, 刘先国. 脊髓背角Ⅱ板层长时程增强诱导及维持过程中的突触形态计量学研究[J]. 生理学报 2004; 56 (3): .

Xiang Xuying, Zhang Hongmei, Hu Nengwei, Zhou Lijun, Zhang Tong, Liu Xianguo. A quantitative study of the synaptic alterations in spinal dorsal horn during the induction and maintenance of long-term potentiation. Acta Physiol Sin 2004; 56 (3): (in Chinese with English abstract).