过刊浏览

缺血预处理合并低温及晶体停搏液对兔未成熟心肌的保护作用

朱珊珊, 张中明, 张育才, 许鹏程, 董红燕, 范建伟, 曾因明

徐州医学院江苏省麻醉医学研究所. 徐州 221002；徐州市第三人民医院麻醉科. 徐州 221002

摘要

本文拟探讨缺血预处理(ischemic preconditioning,IP)合并低温及晶体停搏液对幼兔的离体心脏是否具有心肌保护作用.采用Langendorff离体心脏灌注模型,灌注液为Krebs-Henseleit液(K-H液).取3～4周龄幼兔心脏,在第一部分实验中分为Con、IP1、IP2、IP3组(n=6),分别给予0、1、2、3次IP,其后各组心脏均在20℃低温下停灌2 h,37℃常温下再灌注30 min.在第二部分实验中分为SConI、SCon2、SCon3、SIPl、SIP2、SIP3组(n=8),其中SIPl、SIP2、SIP3组给予2次IP后灌注St.Tho-mas Ⅱ晶体停搏液(CCS)使心脏停搏,然后分别使心脏在32℃、25℃、20℃下停灌30、90和120min,其后各组均在37℃再灌注30 min.SConl,SCon2,SCon3三组则不给予IP,继续灌注20min后灌注CCS使心脏停搏,然后分别在32℃、25℃、20℃下停灌30、90和120 min,其后各组均在37℃再灌注30 min。

关键词： 心肌保护; 缺血预处理; 心肌; 低温

Myocardioprotective effects of the combination of ischemic preconditioning with hypothermia and crystalloid cardioplegia in immature rabbits

Zhang Zhongming, Zhang Yucai, Xu Pengxheng, Dong Hongyan, Fan Jianwei, Zeng Yinming

Jiangsu Institute of Anesthesiology, Xuzhou Medical College, Department of Anesthesiology, The Third Hospital of Xuzhou City. Xuzhou 221002；Department of Thoracic and Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical College. Xuzhou 221002, China

Abstract

This study was undertaken to explore the myocardioprotective effects of the combination of ischemic preconditioning (IP) with hypothermia and St. Ⅱ Thomas crystalloid cardioplegic solution (CCS) on immature hearts in the rabbit. Isolated immature rabbit hearts were perfused with Krebs-Henseleit bicarbonate buffer on Langendorff apparatus. In experiment 1, 24 hearts were divided into 4 groups (n=6 in each group): Con, IP1, IP2 and IP3 group. Hearts of the four groups underwent 0, 1, 2 or 3 cycles of IP respectively. Then all the hearts were subjected to a sustained ischemia period of 2 h at 20℃ and a postischemic reperfusion period of 30 min at 37℃. In experiment 2, 48 hearts were divided into 6 groups (n=8 in each group): SConl, SIP1, SCon2, SIP2, SCon3 and SIP3 group, according to hypothermia and the duration of sustained ischemia (30 min at 32℃; 90 min at 25℃, 2 h at 20℃). The SIP1, SIP2 and SIP3 groups were preconditioned twice before the sustained hypothermic ischemia, while the SConl, SCon2 and SCon3 groups were not preconditioned.

Key words: Ischemic preconditioning;Myocardium;Hypothermia;

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

朱珊珊, 张中明, 张育才, 许鹏程, 董红燕, 范建伟, 曾因明. 缺血预处理合并低温及晶体停搏液对兔未成熟心肌的保护作用[J]. 生理学报 2004; 56 (3): .

Zhang Zhongming, Zhang Yucai, Xu Pengxheng, Dong Hongyan, Fan Jianwei, Zeng Yinming. Myocardioprotective effects of the combination of ischemic preconditioning with hypothermia and crystalloid cardioplegia in immature rabbits. Acta Physiol Sin 2004; 56 (3): (in Chinese with English abstract).