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Caveolin-1蛋白在17β-雌二醇抑制内皮素-1诱导血管平滑肌细胞增殖中的作用

谈智, 林桂平, 王庭槐

中山大学基础医学院生理学教研室. 广东, 广州 510089

摘要

本工作旨在研究Caveolin-1在17β-雌二醇(17β-estradiol,E2)抑制内皮素-1(endothelin-1,ET-1)诱导血管平滑肌细胞(vascular smooth muscle cells,VSMCs)增殖中的作用.在培养的VSMCs上,使用[3H]Thymidine([3H]TdR)掺入法、荧光免疫组化方法和Western blot观察E2处理VSMCs前后ET-1对DNA合成和caveolin-1蛋白表达的影响。

关键词： caveolin-1蛋白; 细胞质膜微囊; 内皮素; 血管平滑肌细胞; 17β-雌二醇

Possible involvement of caveolin-1 in the inhibition of endothelin-1 induced proliferation of vascular smooth muscle cells by 17 #beta#-estradiol

Tan Zhi, Lin Guiping, Wang Tinghuai

Department of Physiology, College of Basic Medical Sciences, Sun Yat-Sen University. Guangzhou 510089, China

Abstract

The aim of the present study was to investigate the rold of caveolin-1 in the inhibition of endthelin-1 induced proliferation of vascular smooth muscle cells (VSMCs) by 17#beta#-estradiol. In the cultured rat thoracic aortic VSMCs, proliferation of VSMCs was determined by using [~(H)]-thymidine incorporation and the expression of caveolin-1 protein was measured by immunofluorescence assays and Western blotting.

Key words: Caveolin-1;Caveolae;Engothelin-1;17#beta#-estradiol;Vsacular smooth muscle cells

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引用本文：

谈智, 林桂平, 王庭槐. Caveolin-1蛋白在17β-雌二醇抑制内皮素-1诱导血管平滑肌细胞增殖中的作用[J]. 生理学报 2004; 56 (3): .

Tan Zhi, Lin Guiping, Wang Tinghuai. Possible involvement of caveolin-1 in the inhibition of endothelin-1 induced proliferation of vascular smooth muscle cells by 17 #beta#-estradiol. Acta Physiol Sin 2004; 56 (3): (in Chinese with English abstract).