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GABA介导杏仁外侧核对皮层A Ⅰ区神经元声反应的抑制:在体微电泳研究

何德富, 陈福俊, 周绍慈

华东师范大学环境科学系. 上海 200062；华东师范大学脑科学研究中心. 上海 200062

摘要

在SD大鼠上应用多管微电极方法,观察微电泳GABA及其受体的拮抗剂或激动剂对杏仁外侧核(LA)抑制皮层A-Ⅰ神经元声反应效应的影响.结果显示,电泳GABA能抑制皮层A-Ⅰ区神经元的电活动,电泳GABAA受体拮抗剂bicuculline(BIC)则能易化其反应;电刺激LA能抑制皮层A I区听神经元声反应,电泳GABA产生类似于刺激LA的抑制效应;LA对皮层A-Ⅰ区神经元的抑制效应能被BIC所翻转,而不能被甘氨酸受体拮抗剂strychnine所翻转,电泳GABAB型受体激动剂bac1ofen对神经元声反应无影响.上述结果表明,GABA可能是介导LA抑制皮层A Ⅰ区神经元声反应的最终递质,并且是通过GABAA受体起作用的。

关键词： 杏仁外侧核; 皮层A-Ⅰ区; 声反应; 抑制; GABA

GABA mediaties the inhibitory effect of lateral amygdaloid nucleus stimulation on the acoustic response of neurons in A Ⅰ cortex:An in vivo microiontophoretic study

He Defu, Chen Fujun, Zhou Shaoci

Department of Environmental Science and, Brain Science Research Center, East China Normal University. Shanghai 200062, China

Abstract

Experiments were performed on Sprague Dawley rats with multibarrel microelectrode technique. The effects of acoustic response of A-Ⅰ cortex neurons produced by electrical stimulation of lateral amygdaloid nucleus (LA) and the influence of GABA were observed. Experimental results showed that iontophoretic administration of GABA caused a pronounced inhibition of the electrical activity of A-Ⅰ neurons. Blockade of GABAA with bicuculline (BIC) facilitated the acoustic response. The acoustic response of A-Ⅰ neurons was inhibited when the LA was stimulated. Iontophoretic application of GABA resulted in a similar inhibitory effect as that of LA stimulation. Blockade of GABAA with bicuculline reversed the inhibitory effect of LA stimulation on the acoustic response of A-Ⅰ neurons. In contrast, application of strychnine, a glycine receptor antagonist, could not reverse the inhibitory effect of LA. Baclofen, a GABAB agonist, did not affect the acoustic response of the auditory neurons. These results indicate that GABA is the ultimate transmitter which mediates the LA stimulation-induced inhibition of the acoustic response of A-Ⅰ neurons in rats, possibly via the GABAAreceptor

Key words: Lateral amygdaloid nucleus;A-Ⅰ cortex;Acoustic response;Inhibition;GABA

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引用本文：

何德富, 陈福俊, 周绍慈. GABA介导杏仁外侧核对皮层A Ⅰ区神经元声反应的抑制:在体微电泳研究[J]. 生理学报 2004; 56 (3): .

He Defu, Chen Fujun, Zhou Shaoci. GABA mediaties the inhibitory effect of lateral amygdaloid nucleus stimulation on the acoustic response of neurons in A Ⅰ cortex:An in vivo microiontophoretic study. Acta Physiol Sin 2004; 56 (3): (in Chinese with English abstract).