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c-myb对人绒毛膜促性腺激素诱导的大鼠睾丸间质细胞睾酮分泌的影响

肖爱娇, 王晶磊, 方廉, 况海斌

江西医学院生理学教研室. 江西, 南昌 330006

摘要

采用离体细胞体外孵育法,观察反义c-myb寡脱氧核苷酸(oligodeoxynucletides,ODN)对人绒毛膜促性腺激素(human chori-onic-gonadotropin hormone,hCG)诱导的大鼠间质细胞睾酮分泌的影响,并进一步探讨了外源性二丁酰cAMP(dbcAMP)、Ca~(2+)及蛋白质抑制剂放线菌酮(cycloheximide,CYX)对间质细胞中c-Myb蛋白表达和睾酮分泌的作用。

关键词： 间质细胞; c-myb; 反义寡脱氧核苷酸; 睾酮

Effect of c-myb on hCG-induced testosterone secretion in isolated rat Leydig cells

Xiao Aijiao, Wang Jinglei, Fang Lian, Kuang Haibin

Department of Physiology, Jiangxi Medical College. Nanchang 330006, China

Abstract

The present study was carried out to investigate the effect of antisense c-myb oligodeoxynucleotides (ODN) on hCG-induced testosterone secretion in isolated rat Leydig cells. The eddects of cAMP, Ca~(2+) and cycloheximide (CYX) on c-Myb protein expression and testosterone secretion were also observed. The results showed that antisense c-myb ODN inhibited hCG-induced testosterone secretion of isolated rat Leyding cells in a dose-dependent manner.

Key words: Leydig cells;c-myb;Antisense oligodeoxynucleotides;Testosterone

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引用本文：

肖爱娇, 王晶磊, 方廉, 况海斌. c-myb对人绒毛膜促性腺激素诱导的大鼠睾丸间质细胞睾酮分泌的影响[J]. 生理学报 2004; 56 (3): .

Xiao Aijiao, Wang Jinglei, Fang Lian, Kuang Haibin. Effect of c-myb on hCG-induced testosterone secretion in isolated rat Leydig cells. Acta Physiol Sin 2004; 56 (3): (in Chinese with English abstract).