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FRIL 蛋白体外维持脐血造血干/祖细胞的作用及细胞周期调控基因的表达

谢小燕, 谢超, 师伟, 李锦, 李艳华, 王冬梅, 白慈贤, 陈琳, 裴雪涛

军事医学科学院输血研究所. 北京 100850

摘要

为探讨新的豆类凝集素(Flt3 receptor-interactinglectin,FRIL)体外维持脐血CD34+细胞的作用以及维持过程中细胞周期调控基因HTm4及HTm4S mRNA的表达及意义,我们利用FRIL维持培养脐血CD34+细胞,对其增殖曲线、细胞周期及集落形成能力进行常规分析,并用半定量RT-PCR法分别测定FRIL体外维持不同时间后脐血CD34+细胞中周期调控基因HTm4及HTm4S mRNA的表达变化。

关键词： 细胞生物学; 造血干/祖细胞; FRIL蛋白; 细胞周期调控; 半定量RT-PCR; HTm4基因; HTm4S基因

The maintenance of cord blood CD34+ progenitor cells with plant lectin FRIL in vitro and the expression of related cell cycle modulator HTm4 and HTm4S

Xie Xiaoyan, Xie Chao, Shi Wei, Li Jin, Li Yanhua, Wang Dongmei, Bai Cixian, Chen Lin, Pei Xuetao

Department of Stem Cell Biology, Beijing Institute of Transfusion Medicine. Beijing 100850, China

Abstract

Exivivo maintainande of human stem cells is crucial for many clinical applications. Current culture conditions provide some level support but cytokines induce most quiescent stem cells to proliferate and differentiate. Better control of primitive is needed to extend the time and range of manipulatin of such cells. A recently identified plant lectin Flt3 receptor interacting lectin (FRIL) present may a special ability to preserve primitive CB progenitors for extended periods in culture without exogenous cytokines.

Key words: Cell biology;Hematopoietic progenitor cells;Cells cycle regulation;FRIL protein;Semi-quantitative RT-PCR;HTm4 gene;HTm4S gene

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引用本文：

谢小燕, 谢超, 师伟, 李锦, 李艳华, 王冬梅, 白慈贤, 陈琳, 裴雪涛. FRIL 蛋白体外维持脐血造血干/祖细胞的作用及细胞周期调控基因的表达[J]. 生理学报 2004; 56 (3): .

Xie Xiaoyan, Xie Chao, Shi Wei, Li Jin, Li Yanhua, Wang Dongmei, Bai Cixian, Chen Lin, Pei Xuetao. The maintenance of cord blood CD34+ progenitor cells with plant lectin FRIL in vitro and the expression of related cell cycle modulator HTm4 and HTm4S. Acta Physiol Sin 2004; 56 (3): (in Chinese with English abstract).