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不同诱导因子对人外周血单个核细胞P2X7受体表达的作用

张秀军, 郑国光, 马小彤, 林永敏, 宋玉华, 吴克复

医科院,中国协和医科大学,血液学研究所,实验血液学国家重点实验室.天津 300020

摘要

ATP激活P2X7受体可产生一系列的白细胞功能反应,因此P2X7受体的表达调控引起我们的兴趣。然而P2X7受体在正常人外周血单个核细胞(peripheral blood mononuclear cells,PBMC)、单核细胞中的表达调控机制尚未阐明。该文用半定量RT--PCR方法检测多种细胞因子、细菌抗原、丝裂原对P2X7受体表达的诱导作用,探索P2X7受体的诱导表达模式。结果表明,单个核细胞和单核细胞可检出P2X7受体的表达;白细胞介素2、4、6(interleukin--2、--4、--6,IL--2、IL--4、IL--6)、肿瘤坏死因子#alpha#(tumour necrosisfactor--#alpha#,TNF--#alpha#)等细胞因子和金黄色葡萄球菌Cowan Ⅰ株(Staphylococcus aureus Cowanstrain Ⅰ,SAC)、脂多糖(lipopolysaccharide,LPS)能上调PBMC的P2X7受体表达,而#gamma#干扰素(interferon--#gamma#,IFN--#gamma#)、粒--巨噬细胞集落刺激因子(granulocyte--macrophage colony--stimulating factor,GM--CSF)、巨噬细胞集落刺激因子(macrophage colony--stimu--latingfactor,M--CSF)和植物血凝素(phytohemagglutinin--M,PHA--M)等则没有作用;LPS和M-CSF可以提高单核细胞的P2X7受体表达,IFN--#gamma#、TNF--#alpha#、GM--CSF作用较弱,但是这些因子的预处理并不能增强LPS对P2X7受体表达的诱导。炎症因子促进P2X7受体的表达,提示P2X7受体可能在对抗细菌感染的免疫反应中起一定作用,这有待于进一步研究。

关键词： P2X7受体; 细胞因子; 外周血单个核细胞; 单核细胞

Effects of various inducers on the expression of P2X7 receptor in human peripheral blood mononuclear cells

Zhang Xiujun, Zheng Guoguang, Ma Xiaotong, Lin Yongmin, Song Yuhua, Wu Kefu

State Key Laboratory for Experimental Hematology,Institute of Hematology,Chinese Academy of Medical Sciences and Peking Union Medical College.Tianjin 300020

Abstract

Regulation of P2X7 receptor expression isof interest because activation of this receptor by extracellular ATP triggers a wide variety of cell functions in leukocytes. However, its expression and modulation in human peripheral blood mononuclear cells (PBMC)and monocytes remain unclear. RT-PCR was used to detect the constitutive level of P2X7 receptor and the levels upon stimulation with bacteria, bacterial product, mitogen and various cytokines in human PBMC and monocytes. P2X7 receptor mRNA was detected in PBMC and monocytes. P2X7 receptor expression in PBMC was up-regulated by interleukin-2, -4, -6 (IL-2, IL-4, IL-6) tumour necrosis factor-#alpha# (TNF-#alpha#), lipopolysaccharide (LPS) and heat-inactivated Staphylococcus aureus Cowan strain Ⅰ (SAC). However,interferon-#gamma# (IFN-#gamma#), granulocyte-macrophage colony-stimulating factor (GM-CSF), macrophage colony-stimulating factor (M-CSF)and phytohemagglutinin-M (PHA-M) had little effect on the expression of P2X7 receptor. Furthermore, LPS and M-CSF could upregulate P2X7 receptor expression in monocytes, while IFN-#gamma#, TNF-#alpha# and GM-CSF had weak effects, but pretreatment with these inducers could not further enhance LPS-stimulated P2X7 receptor expression in monocytes. The results obtained demonstrate that inflammatory stimuli drive P2X7 expression, thus supporting the hypothesis that P2X7 receptor may play a role in the inflammatory responses against bacteria infection, which need further verification.

Key words: P2X7 receptor;cytokines;peripheral blood mononuclear cells;monocytes

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引用本文：

张秀军, 郑国光, 马小彤, 林永敏, 宋玉华, 吴克复. 不同诱导因子对人外周血单个核细胞P2X7受体表达的作用[J]. 生理学报 2005; 57 (2): .

Zhang Xiujun, Zheng Guoguang, Ma Xiaotong, Lin Yongmin, Song Yuhua, Wu Kefu. Effects of various inducers on the expression of P2X7 receptor in human peripheral blood mononuclear cells. Acta Physiol Sin 2005; 57 (2): (in Chinese with English abstract).