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组织学与核磁共振检测大鼠癫痈源性早期脑损伤的跨半球扩布特征

韩丹, 减颖, 杨运煌, 刘买利, 王文挺, 邹祖玉

武汉大学医学院生理学系、牛国科学院武汉物理与数学研究所波谱与原子分子物理国家院病理学与病理生理学系，武汉430071

摘要

慢性强直电刺激(60 Hz, 2 s)大鼠右侧背海马(hippocampus , DHPC) CAI基树突区，1次/d，连续刺激10 d分别在施加强直电刺激的第2,4,6,8或10 d时进行核磁共振成像检测(Tz weighted magnetic resonance image , T2- WI) ,并对鼠脑进行组织学切片鉴定。结果表明，早期慢性癫痈源性脑损伤的病理性形态特征主要包括:(1) T_(2)- WI检测侧
脑室((lateral ventricle, LV)区域信号增强、组织学检测LV扩大和双侧对称性脉络膜丛病理性增生，后两者并非完全平行呈现。(2)组织学切片显示双侧LV扩大面积与T2- WI信号增强区域面积的脑区分布近似。与空白对照组大鼠相比，电刺激2.4.6.8和10 d后，T2- WI信号增强区域面积显著增大(P=0.0259 ; P=0.0184; P=0.0184; P=0. 0404 ; P=0.0259)以及组织学鉴定LV面积增大(P=0. 0210 ; P=0.01;P=0. 01 00 ; P=0. 0152)。(3)定侧分析显示，T2- WI信号增强以及T_(2)- WI信号增强区域面积和组织学鉴定LV面积扩大，在慢性刺激6 d时均以植入电极的对侧为主;第10 d时均以同侧为主。三项观察结果的一致性证实了癫痈源性早期脑损伤的跨半球动态扩布特征。

关键词： 脑; 72加权核磁共振; 侧脑室扩大; 癫痈; 大鼠

Propagation of brain i对uries from artificial focts into the opposite hemisphere at the early stage of rat electrogenic epilepsy identified by histology and magnetic resonance image

Han Dan, ZANG Ying, Yang Yunhuang, LIU Mai- Lie, Wang Wenting, Zou Zuyu

Department of Physiology and Pathology and Pathophysiology, Medical College , Wuhan University Wuhan 430071；Laboratory of Magnetic Atomic Resonce and Molecular Physics , Wuhan Institute of Physics and Mathematics，Chinese Academy of Sciences，Wuhan 430071

Abstract

The popurse of this work was to study the characteristics of rat brain abnormalities at two he spheres at the early stage of electrogenic epilepsy .Experiments were performed on 37 male Sprague- Dawleyra Chronically repetitive tetanization(60 Hz，2s，0.4一0. 6 mA) was used to stimulate the right dorsal hippoca pus(DHPC) of the rat brain once a day for 2，4，6，8 or 10 d，respectively .The weighted magnetic renance image(T_(2) WI) were obtained from each experimental rat at the end of the xperiments .Histological si tions were obtained after experimentation .The results showed that the main pathologic changes at the early st;of epilepsy included:(1)T_(2) WI hype rintensification，the histological enlargement of lateral ventricle choroidea plaxus pathological perplasia of ventricular choroidea plexus occurred .The pathological hyperplasia was symmetric two hemispheres，but the LV enlargement was not.(2) Histologically enlarged LV area showed a resemblance T_(2) WI hyperintensive area .Compared with the control rats，large T_(2)WI hyperintensive area(P一0.0259;P一0.0184;P一0.0184;P一0.0404;P一0. 0259) and histologically enlarged LV area(P一0.0210;P一0.01P一0.0100;P一0. 0152) were present in chronically tetanized rats.(3)Dynamic characteristics of histologi-
cally enlarged LV area resembled to those of T_(2) WI hyperintensity area in chronically tetanized rats at differen stimulating day .Lateralization of T_(2)WI hyperintensity was in accordance with that of T_(2) WI abnormal area and o
histologically enlarged LV.These abnormalities were severe on the ontralateral side on the stimulating day 6.or on the ipsilateral side on the stimulating day 10 .These results imply characteristic propagation of brain abnormalities crossing to the opposite hemisphere at the early stage of an electrogenic rat epilepsy.

Key words: brain;magnetic resonance imaging;lateral ventricle;epilepsy;rats

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引用本文：

韩丹, 减颖, 杨运煌, 刘买利, 王文挺, 邹祖玉. 组织学与核磁共振检测大鼠癫痈源性早期脑损伤的跨半球扩布特征[J]. 生理学报 2003; 55 (3): .

Han Dan, ZANG Ying, Yang Yunhuang, LIU Mai- Lie, Wang Wenting, Zou Zuyu. Propagation of brain i对uries from artificial focts into the opposite hemisphere at the early stage of rat electrogenic epilepsy identified by histology and magnetic resonance image. Acta Physiol Sin 2003; 55 (3): (in Chinese with English abstract).