过刊浏览

胚胎神经干细胞移植及胶质细胞源性神经营养因子对大鼠脊髓损伤的修复作用

孙勇, 侍坚, 富赛里, 陆佩华, 徐晓明

生海第二医科大学神经生物学实验室，上海200025；美国肯塔基脊髓损伤研究中心，美国神经外科，路易维尔市，肯塔基州40292，美国

摘要

将胚胎神经干细胞(neural stem cells, NSCs)移植至成年大鼠损伤的脊髓，观察移植后NSCs的存活、迁移以及损伤后的功能恢复。实验结果显示:动物NS Cs移植4周后，斜板实验平均角度和运动评分结果比对照组均有明显增高(P<0.05)，而脊髓损伤(spinal cord injury , SCI)处的空洞面积显著减小(P<0.05) ;在NS Cs中加入胶质细胞源
性的神经营养因子(glial cell line- derived neurotrophic factor , GDNF)后，上述改变更加显著。移植后的NS Cs不仅能存活，而且向损伤的头端和尾端迁移达3 mm之远。这些结果表明，移植的NS Cs不仅可以存活、迁移，还可减小SCI空洞面积，促进动物神经功能的恢复;此外，我们的结果还表明GDNF对SCI功能恢复有促进作用。

关键词： 脊髓损伤; 胚胎神经干细胞; 移植; 胶质细胞源性的神经营养因子

Effects of embryonic neural stem cells and glial cell line derived neurotrophic factor in the repair of spinal cord i对ury

Sun Yong, Shi Jian, FU Sai- Li, LU Pei- Hua, XU Xiao- Mng

Departnrnt of Neurobiology , Shanghai Second Medical University, Shanghai 200025 ； Kentucky Spinal Cord In-jury Research Center，Department Neurological Surgery，University of Louisville，511 S. KY40292，USA

Abstract

The ability of implanted embryonic neural stem cells(NSCs) to improve survival，migration，and functional recovery following a compression spinal cord injury(SCI) was tested in adult rats.NSCs were isolated
from E14-16 rat cerebral cortex and SCI was produced妙using an aneurysm clip applicator applied to the 8th thoracic spinal cord according to method‘Dolan and Tator .Two weeks after the injury，NS Cs(4 u1‘1/10'
cells/ pJ) were injected into the lesion site.，The grafted NSCs were noted to survive and integrate with the host spinal cord 1 month after transplantation，which was demonstrated妙the presence of Hoechst 33342(a nuclear
dye) pre-labeled NSCs within and surrounding the lesion site .Some of these cells remained undifferentiated and were stained with nestin，a marker for NSCs .Transplanted NSCs migrated for at least 3 mm from the injury epi-
center towards both the rostral and caudal directions .Significant reduction in the lesion area(P<0. 05) and improvement in inclined plane(P<0. 05) and BBB locomotor rating scale(P<0. 05) were found in the cases that received implantation of NSCs，as compared with those that received vehicle .More importantly，when glial cell line-derived neurotrophic factor(GDNF;1 .5 pg/ pJ) was added to the transplants，further reduction in

Key words: spinal cord injury;;;

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

孙勇, 侍坚, 富赛里, 陆佩华, 徐晓明. 胚胎神经干细胞移植及胶质细胞源性神经营养因子对大鼠脊髓损伤的修复作用[J]. 生理学报 2003; 55 (3): .

Sun Yong, Shi Jian, FU Sai- Li, LU Pei- Hua, XU Xiao- Mng. Effects of embryonic neural stem cells and glial cell line derived neurotrophic factor in the repair of spinal cord i对ury. Acta Physiol Sin 2003; 55 (3): (in Chinese with English abstract).