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#beta#_(2)肾上腺素受体在新生大鼠心肌成纤维细胞中的分布及其促增殖作用

尹峰, 吕志珍, 韩启德, 张幼怡

北京大学第三医院血管医学研究所，分子心血管学教育部重点实验室，北京100083

摘要

为了明确分肾上腺素受体(AR)亚型在新生大鼠心肌成纤维细胞中的分布及其在成纤维细胞增殖反应中的作用，采用放射配体结合实验和「3 H ]-thymidine掺入法检测了新生大鼠心肌成纤维细胞的[r AR密度和DNA合成速率。结果显示，在培养心肌细胞和心肌成纤维细胞中[3 AR密度(Bmaa)和解离常数(场)无显著性差异;竞争抑制曲线分析结果提示，心肌成纤维细胞对CGP 20712A和ICI 118551单位点拟合均显著优于两位点拟合(P<0.01)，表现为对选择性[3l- AR拮抗剂CGP 20712A的低亲和性(I C;。值:10. 1 umol/ L)和对选择性[3z AR拮抗剂ICI 118551的高亲和性(Iq。值:0.147 11 mol/ L)。异丙肾上腺素(ISO)促心肌成纤维细胞增殖作用可被ICI 118551和心得安(非选择性[r AR拮抗剂)完全抑制，而CGP 20712 A则无此作用。上述结果提示，在培养心肌成纤维细胞中[3z AR亚型占绝对优势，并且ISO引起的心肌成纤维细胞增殖反应是由[32 AR介导的。

关键词： 分肾上腺素受体; 放射配体结合实验; 心肌成纤维细胞; 增殖

Expression of #beta#_(2) adrenergic receptor and its effect on the proliferation of neonatal rat cardiac fibroblasts

Yin Feng, LU Zhi- Zhen, Han Qide, ZHANG You Yi

Institute of Vascular Medicine，Peking Unizrrsity Third Hospital，The Reference Laboratory of Ministry of Education for Moleculer Cardiovasology，Beijing 100083-

Abstract

The expression of f}-adrenergic receptor subtypes and its effect on neonatal rat cardiac fibroblast proliferation were investigated妙radioligand binding assay and[3 H rthymidine incorporation analysis，respec-
tively .The results indicated that there was no significant difference in the f}-adrenergic receptor density(Bmax)and affinity(KD) between cardiomyocytes and cardiac fibroblasts.The[~(125)I rpindolol competitive inhibition
curves(ICI 118551 and CGP 20712A) were significantly better fit in a one-site model in membrane preparation of cardiac fibroblasts .in cultured cardiac fibroblasts，0. 1 p mol/ L isoproterenol-induced[~(3)Hthymidine incor
poration was completely inhibited by a selective O2 AR antagonist ICI 118551，or a non- selective f}- AR antagonist propranol of，but not妙CGP 20712A，a selective p,一AR antagonist .These results suggest that isoproterenol-in-
duced cardiac fibroblast proliferation is mediated妙p2 AR，the preponderant f- AR subtype in cardiac fibroblasts.

Key words: adrenergic receptor;radioligand binding assay;Cardiac fibroblasts;proliferation

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引用本文：

尹峰, 吕志珍, 韩启德, 张幼怡. #beta#_(2)肾上腺素受体在新生大鼠心肌成纤维细胞中的分布及其促增殖作用[J]. 生理学报 2003; 55 (3): .

Yin Feng, LU Zhi- Zhen, Han Qide, ZHANG You Yi. Expression of #beta#_(2) adrenergic receptor and its effect on the proliferation of neonatal rat cardiac fibroblasts. Acta Physiol Sin 2003; 55 (3): (in Chinese with English abstract).