过刊浏览

一套研究机械电反馈的心室压力钳系统

魏华, 黄海霞, 王伟, 张知非, 付小锁, 刘萍, 钮伟真

首都医科大学生理学系.北京 100069

摘要

在心脏机械电反馈的研究中准确控制机械刺激是非常重要的。该研究室构建了一套适用于离体家兔心脏的心室压力钳系统。该系统通过计算机控制压力钳，不仅能模拟正常生理条件下左心室的压力波形，还能在心室活动周期的特定时相、以适当波形对心室施加机械刺激。该系统集心脏灌流与起搏、表面心电图记录、单相动作电位记录、心室压力钳制与测定等多种功能于一体，特别适用于器官水平上观察机械电反馈现象并探讨其机制。

关键词： 压力钳; 机械电反馈; 心室; 心脏灌流

A ventricular pressure--clamping system for the study of mechano--electrical feedback

Wei Hua, Huang Haixia, Wang Wei, Zhang Zhifei, Fu Xiaosuo, Liu Ping, Niu Weizhen

Department of Physiology, Capital Medical University.Beijing 100069

Abstract

It is necessary to control the mechanical stimuli precisely in the studies of cardiac mechano-electrical feedback （MEF）. In the present study a ventricular pressure-clamping system has been developed, which can be applied to isolated-perfused rabbit hearts. Controlled by a computer, this system not only can make the left ventricle follow a command defining the same pressure wave as that during a beating cycle under physiological condition, but also deliver mechanical stimuli with a proper waveform to the ventricle at a particular time phase. This system integrates multiple functions, including perfusing, pacing, recording of electrocardiogram and monophasic action potentials, and clamping and measuring of ventricular pressures in isolated-perfused hearts. Thus, it is a distinct system for investigating the phenomena and mechanisms of cardiac MEF at organ level.

Key words: pressure-clamp;mechano-electrical feedback;ventricle;perfusing heart

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

魏华, 黄海霞, 王伟, 张知非, 付小锁, 刘萍, 钮伟真. 一套研究机械电反馈的心室压力钳系统[J]. 生理学报 2006; 58 (6): .

Wei Hua, Huang Haixia, Wang Wei, Zhang Zhifei, Fu Xiaosuo, Liu Ping, Niu Weizhen. A ventricular pressure--clamping system for the study of mechano--electrical feedback. Acta Physiol Sin 2006; 58 (6): (in Chinese with English abstract).