过刊浏览

一氧化氮对呼吸节律性放电的调节作用

李自强, 吴中海, 史玥, 王宁黔　

第一军医大学生理教研室. 广州 510515

摘要

实验旨在探讨一氧化氮(nitric oxide,NO)在基本呼吸节律产生和调节中可能的作用.制作新生大鼠离体延髓脑片标本,主要包含面神经后核内侧区,前包钦格复合体、腹侧呼吸组以及背侧呼吸组的一部分.同时保留舌下神经根,用改良Kreb's液灌流脑片并记录与之相连的舌下神经根呼吸节律性放电(respiratory rhmical dischargeactivity,RRDA),在灌流液中分别给予不同浓度的NO供体硝普钠(sodium nitroprusside,SNP),NO合成前体L-精氨酸(L-Arrginine,L-Arg)以及神经元型一氧化氮合酶(neuronal nitric oxide synthase,nNOS)特异性抑制剂7-nitro indazole(7-MI),观察其对RRDA的影响.结果显示,nNOS的特异性抑制剂7-NI对吸气时程和放电强度有明显抑制,而NO合成前体L-Arg,以及NO供体SNP对呼吸放电活动没有明显的影响.这提示,在哺乳动物基本呼吸节律的产生和调节中,NO可能对吸气中止和呼吸幅度具有调节作用。

关键词： 一氧化氮; 面神经后核内侧区; 前包钦格复合体; 呼吸节律; 一氧化氮合酶

Nitric oxide is involved in the modulation of central respiratory rhythm

Li Ziqiang, Wu Zhonghai, Shi Yue, Wang Ningqian

Department of Physiology, The First Military Medical University. Guangzhou 510515, China

Abstract

This experiment was expected to test whether nitric oxide (NO) exerted significant effect onthe central respiratory rhythm. Experiments were performed on in vitro brainstem slice preparations fromneonatal rats. These preparations include the medial region of the nucleus retrofacialis (mNRF); a part ofpre-Botinger complex, ventral respiratory group (VRG) and dorsal respiratory group (DRG). Respiratory-related burst activities were recorded from hypoglossal nerve rootlets before and during superfusion of theslice preparation with L-Arginine (L-Arg), sodium nitroprusside (SNP) or 7-nitro indazole (7-NI, an in-hibitor of NO synthase). After perfusion with L-Arg and SNP, there was no significant change in respiratoryrhythmical discharge activity (RRDA), but 7-NI decreased the integral amplitude of burst and inspiratorytime. These results indicate that NO may take part in the inspiratory off-switching mechanism and that it al-so modulates the amplitude of respiratory-related bursts.

Key words: Nitric oxide the medial region of the nucleus retrofacialis;Pre-Botinger complex;Respiratory rhythm;Nitric oxide synthase ;

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

李自强, 吴中海, 史玥, 王宁黔　 . 一氧化氮对呼吸节律性放电的调节作用[J]. 生理学报 2003; 55 (5): .

Li Ziqiang, Wu Zhonghai, Shi Yue, Wang Ningqian. Nitric oxide is involved in the modulation of central respiratory rhythm. Acta Physiol Sin 2003; 55 (5): (in Chinese with English abstract).