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肾动脉内注射L一精氨酸抑制肾神经传入纤维的自发活动

马慧娟, 刘宜先, 武宇明, 何瑞荣

河北医科大学基础医学研究所生理室，石家庄 050017

摘要

研究旨在应用记录肾传入神经多单位和单位放电的方法，观察肾动脉内注射L一精氨酸对麻醉家兔肾神经传入纤维自发放电活动的影响。结果表明:(1)肾动脉内注射L一精氨酸((0. 05,0.24和。.48 m mol/ kg)可呈剂量依赖性地抑制肾传入纤维的活动，而动脉血压不变;(2)静脉内预先注射一氧化氮合酶抑制剂I- NAME (0.11 m mol/ kg)，可完全阻断L-精氨酸对肾传入纤维的抑制;(3)‘肾动脉注射一氧化氮(NO)供体SI N1 (3.75 umol/ kg)也可抑制肾传入神经的活动。以上结果提示:‘肾动脉内应用NO前体L-精氨酸和NO供体SI N 1均可抑制肾传入纤维的自发活动。

关键词： 生理学; L一精氨酸; 单位放电; 肾传入神经

I rntrarenal artery injection of L- arginine inhibits spontaneous activity of renal afferent nerve fibers

MA Hui Juan, LIU Yi Xian, WU Yu Ming, HE Rui Rong

Department of Physiology，Institute of Basic Medicine，Hebei Medical University，ShiJiazhuang 050017

Abstract

The purpose of this study was to determine the effect of intrarenal artery injection of L-arginine on multi and single-unit spontaneous discharges of renal afferent nerve fibers in anesthetized rabbits .The results obtained are as follows:(1)intrarenal artery injection of L-arginine(0.05，0. 24，and 0. 48 m mol/ kg) decreased the renal afferent nerve activity (ARNA) in a dose-dependent manner with arterial pressure unchanged;(2) pretreatment with a nitric oxide synthase inhibitor L- NAME(N6-nitro- L-arginine methylester，0.11 m mol/ kg)，completely abolished the effect of L-arginine;and (3) intrarenal artery injection of a nitric oxide donor SIN-1(3- morpholinosydnonimine，3. 75 umol/ kg) also resulted in an inhibition of ARNA.The results suggest that intrarenal artery injection of NO precursor(L-arginine) and donor(SIN-1)
can inhibit ARNA in anesthetized rabbits.

Key words: Physiology;L-arginine;unit- activity;renal afferent nerve

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引用本文：

马慧娟, 刘宜先, 武宇明, 何瑞荣. 肾动脉内注射L一精氨酸抑制肾神经传入纤维的自发活动[J]. 生理学报 2003; 55 (2): .

MA Hui Juan, LIU Yi Xian, WU Yu Ming, HE Rui Rong. I rntrarenal artery injection of L- arginine inhibits spontaneous activity of renal afferent nerve fibers . Acta Physiol Sin 2003; 55 (2): (in Chinese with English abstract).