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肿瘤坏死因子对大鼠中脑神经干细胞分化和寡突胶质细胞增殖的影响

娄淑杰, 顾平, 徐荷, 徐晓辉, 王铭维, 何成, 路长林

第二军医大学神经生物学教研室，上海200433；河北医科大学第一医院神经内科，石家庄 50013 ；第二军医大学流行病学教研室，上海200433

摘要

为观察肿瘤坏死因子对神经干细胞(NS Cs)分化的影响，本研究应用体外扩增的新生大鼠中脑NSCs，使用免疫组织化学技术，观察了肿瘤坏死因子一。(TNFa)对NSC、分化及其后代细胞的影响。结果显示:(1) TNFa可提高中脑NS Cs后代中神经元和寡突胶质细胞所占的比例;(2) TNF-。可明显诱导由NS Cs分化的寡突胶质细胞增殖，但对星形胶质细胞的增殖作用不明显。上述观察结果提示TNF-。对NS Cs的应用具有重要影响。

关键词： 肿瘤坏死因子-#alpha#; 神经干细胞; 寡突胶质细胞; 分化

Effect of tumor necrosis factor a on differerntiation of mesencephalic neural stem cells and proliferation of oligodendrocytes in the rat

LOU Shir Jie, GU Ping, XU He , XU Xiao Hui, WANG Ming Wei, He Cheng, Lu Changlin

Department of Neurobiology, Second Military Medical University, Shanghai 200433；Department of Neurology, First Hospital, Medical University of Hebei, Shijiazhuang 050013；Department of Epidemiology，Second Military Medical University，Shanghai 200433

Abstract

To observe the influence of tumor necrosis factora(TNF-a) on differentiation of rat mesencephalic neural stem cells(NS Cs)，the numbers of neurons，astrocytes and oligodendrocytes generated from NSCs were analyzed after differentiation for 3 days by using i m munocytoche mistry technique.The
results show that:(1)TNF-a enhanced the proportions of neurons and endrocytes in progeny of NS Cs;and(2) TNF-a induced the proliferation of oligodendrocytes derived from NSCs，but the prolifer ation of astrocytes was not influenced by TNF-a.We conclude that the TNF-a could influence the application of NSCs.

Key words: neural stem cells;oligodendrocytes;differentiation ;

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引用本文：

娄淑杰, 顾平, 徐荷, 徐晓辉, 王铭维, 何成, 路长林. 肿瘤坏死因子对大鼠中脑神经干细胞分化和寡突胶质细胞增殖的影响[J]. 生理学报 2003; 55 (2): .

LOU Shir Jie, GU Ping, XU He , XU Xiao Hui, WANG Ming Wei, He Cheng, Lu Changlin. Effect of tumor necrosis factor a on differerntiation of mesencephalic neural stem cells and proliferation of oligodendrocytes in the rat. Acta Physiol Sin 2003; 55 (2): (in Chinese with English abstract).