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骨髓基质支持造血功能观察

谢仁敷, 纪树荃, 杜占京, 金淑子, 王树琴, 曹秀珍

中日友好医院；航天部中心医院

摘要

正常人和小鼠骨髓细胞体外平皿液体培养中形成的贴壁细胞层,具有骨髓基质成分和功能,在此贴壁细胞层或基质层上,培养粒系祖细胞 CFU-C(Colony Forming Unit-Culture),以不带基质层的 CFU-C 为对照(100%)。结果第一周基质层上 CFU-C 为对照51.07±3.54%(人)和27.30±4.68%(鼠);第二周者则与对照相近;笫三周之 CFU-C 则上升达150.70±17.63%(人)与146.89±10.01%(鼠)。示基质层对 CFU-C 的增殖分化,在1～3周呈先抑制后刺激的效应。并观察了10例用山莨菪碱治疗的再生障碍性贫血患者之骨髓三周基质层,对正常骨髓CFU-C 影响。

关键词： 基质层; CFU-C; 造血效应; 山莨菪碱

A preliminary observation on the hemopoietic inductive effect of marrow stroma

Ji Sunquan, Du Zhenging, Jin Shuzi, Wang Shangin, Cao Xouzhen

The Genetal Hospital of the Ministry of Astronautry

Abstract

The adherent cell layer formed by cultivating bone marrow cell in a liquid
system in dishes was taken as a stromal layer (SL) for studying the interaction of the hemopoietic cells and the stroma. The granulocyte-macrophage progenitor
cells (CFU-C) culture system in agar was overplated directly onto the human and mouse marrow SL which has been incubated for 1 to 3 weeks. In comparison with the CFU-C grown without SL as a control, the CFU-C grown on the first week SL were 51.07±3.540o (human) and 27.30±4.6800 (mouse) respectively; on the second week SL, the growth of CFU-C was practically the same; while the sum of the CFU-C colonies overlayed onto the third week SL increased up to 150.70±17.63%(human), and 146.89±10.01% (mouse).The results showed that SL affected granulopoiesis from inhibition to stimulation in a period of 3 weeks. This affection was probably mediated by the colony stimulating factor.

Key words: Stroma layer;CFU-C;Haemopoiesis;Amisodamin

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引用本文：

谢仁敷, 纪树荃, 杜占京, 金淑子, 王树琴, 曹秀珍. 骨髓基质支持造血功能观察[J]. 生理学报 1987; 39 (2): .

Ji Sunquan, Du Zhenging, Jin Shuzi, Wang Shangin, Cao Xouzhen. A preliminary observation on the hemopoietic inductive effect of marrow stroma. Acta Physiol Sin 1987; 39 (2): (in Chinese with English abstract).