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胎肝造血干细胞在扩散盒培养条件下移植特性的变化

魏启胜, 吴祖泽

军事医学科学院放射医学研究所

摘要

经体内扩散盒培养6d 后的 LACA 小鼠胎肝细胞移植给照射的同系成年小鼠,造血干细胞在受体脾脏和骨髓中的有效植入率比正常胎肝细胞明显提高。但这种效果在同种异基因受体小鼠中则完全消失。实验结果表明,个体发育屏障和移植免疫屏障是决定同种胎肝移植能否成功的两个重要因素。胎肝细胞经体内或体外培养后可以模拟造血干细胞在体内的发育成熟,从而增强对成年造血微环境的适应性。用短期体内培养的方法,可以改变胎肝造血干细胞的某些生理特性,从而减弱个体发育屏障,但不能克服胎肝同种移植中的免疫性抗力。

关键词： 胎肝; 造血; 扩散盒; 移植; 干细胞

Changes of transplantation pecurialities of fetal liver hemopoietic stem cells after short-term culture in diffusion chamber in vivo

Wei Qisheng, Wu Zuze

Institute of Radiation Medicine, Academy of Military Medical Science. Beijing, China

Abstract

Fetal liver cells from LACA inbred mice after 6 days of in vivo culture were
transplanted into a group of syngeneic irradiated adult mice, the seeding efficiency of hemopoictic stem cells both in recipient spleen and bone imarrow, in comparing;with the normal fetal liver cells, was obviously increased. This effect was completely abolished in allogencic transplantation of fetal liver cells i:rom LACA mice into.lethally irradiated C6, adult mice. Experimental results showed that ontogenetic and histoincompatibal barriers are the major factors determining the success of allogeneic fetal liver transplantation. In vitro or in vivo culture of fetal liver cells may create favourable conditions to accelerate the development of hemopoietic stem cells from the fetal immature stage to adult mature state, and therefore makes them adaptable to lodge in adult bone marrow microenvironment. The shortterm in vivo culture as reported in this paper showed an effect of transformation of some genetic characters across the ontogenetic barrier but could not overcome the barrier of allogenetic resistance. Hence it is likely that the histoincompatable barrier plays more important role than ontogenetic barrier to ensure the successful engraftment in allogenetic fetal liver transplantation and the final achievement
to this end requires further removal of this obstacle.

Key words: Fetal liver;Hemopoiesis;Diffusion chamber;Transplantation;Stem cells

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引用本文：

魏启胜, 吴祖泽. 胎肝造血干细胞在扩散盒培养条件下移植特性的变化[J]. 生理学报 1987; 39 (2): .

Wei Qisheng, Wu Zuze. Changes of transplantation pecurialities of fetal liver hemopoietic stem cells after short-term culture in diffusion chamber in vivo. Acta Physiol Sin 1987; 39 (2): (in Chinese with English abstract).