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急、慢性乙醇处理后大鼠伏核内cAMP反应元件结合蛋白磷酸化的变化

李菁, 李跃华, 张小虎, 朱学江, 戈应滨, 袁孝如

南京医科大学，病理学与病理生理学系，2生理学系，南京210029

摘要

采用免疫组织化学的方法，检测急性、慢性乙醇作用及戒断后大鼠伏核内cAMP反应元件结合蛋白(CAMP response element binding protein, CREB)磷酸化的变化。结果显示，急性腹腔注射乙醇后15 min，伏核内磷酸化CREB( phospho- CREB, p- CREB)蛋白明显增加，30 min后达高峰，至1和6 h后仍明显高于对照组。而慢性饮乙醇溶液显著降低大鼠伏核内p- CREB蛋白含量，在撤除乙醇后24.72 h时，伏核内p- CREB蛋白含量仍明显较低，戒
断后7 d，恢复到正常水平。结果表明，急性乙醇处理增加伏核内CREB磷酸化作用，而慢性乙醇作用则降低伏核内C REB磷酸化作用，这可能是乙醇依赖的分子机制之一。

关键词： 乙醇; 伏核; cAMP反应元件结合蛋白

Charges in the phosphorylation of cAMP response element binding protein in the rat nuclets accumbers after ache anal chrornc ethaml admirisstration

Li Jing, LI Yue- Hual, ZHANG Xiao Hu, ZHU Xue Jiang, GE Ying Bin, Yuan Xiaoru

(Department of Pathology and Pathophysiology and Department of Physiology, Nanjing Medical University，Nanjing 210029l

Abstract

To define the molecular basis of ethanol dependence，changes in the phosphorylation of cAMP response element binding protein(CREB) in the nucleus accu m阮ns of rats after acute and chronic ethanol administration were detected using immunohistochemist ry.The results demonstrate that the expression of phospho-CREB(p-CREB) protein in the rat nucleus accumbens significantly increased after 15 min of acute ethanol exposure，reaching a peak at 30 min after ethanol administration .The increment remained after 1 or 6 h of ethanol exposure compared to the control rats.In contrast，chronic intake of ethanol solution obviously decreased the expression of p-CREB protein compared to the control rats .The decrement remained 24 h or 72 h after ethanol withdrawal，and returned to the control levels after 7 d of
ethanol withdrawal .The results suggest that an acute ethanol administration led to an increase in the phosphorylation of CREB in the nucleus accumbens，but chronic ethanol administration produced a decreme nt，which is possibly one of the molecular mechanisms of alcohol dependence.

Key words: Ethanol;nucleus accu mbens;cAMP response element binding protein(CREB)

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引用本文：

李菁, 李跃华, 张小虎, 朱学江, 戈应滨, 袁孝如. 急、慢性乙醇处理后大鼠伏核内cAMP反应元件结合蛋白磷酸化的变化[J]. 生理学报 2003; 55 (2): .

Li Jing, LI Yue- Hual, ZHANG Xiao Hu, ZHU Xue Jiang, GE Ying Bin, Yuan Xiaoru. Charges in the phosphorylation of cAMP response element binding protein in the rat nuclets accumbers after ache anal chrornc ethaml admirisstration. Acta Physiol Sin 2003; 55 (2): (in Chinese with English abstract).