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八肽胆囊收缩素对家兔内毒素休克时肺动脉张力的影响

段国辰, 凌亦凌, 谷振勇, 韦鹏, 牛志云, 杨世方

河北医科大学病理生理教研室，石家庄。50017

摘要

为探讨八肽胆囊收缩素(CCK 8)缓解内毒素休克(ES)时肺动脉血压(PAP)增高的机制，观察了CCK 8对脂多糖(LPS)引起家兔ES时PAP变化以及离体肺动脉环(PARs)张力改变的影响。实验用新西兰大耳白雄性家兔40只，分为颈静脉注入LPS (8 mg/ kg i. v.)复制的家兔ES模型、LPS注入前15 min给CCK 8 (15 ug/ kg, i. v. ) ,LPS注入前15 min给CCK受体拮抗剂丙谷胺(Pro1 mg/ kg, i. v.)、单独注入CCK 8 (15 ug/ kg, i. v.)和注射生理盐
水(对照)共5组。用生理记录仪监测平均动脉压(MAP)和PAP的变化;5 h后制备PARs，应用血管张力测定技术，检测各组PARs张力。结果为:(1) ES时MAP降低、PAP升高，CCK 8可完全翻转ES时PAP的增高，而Pr。加剧ES时PAP的增高;(2) LPS组的PARs对苯肾上腺素(PE)的收缩反应增强，对ACh内皮依赖性舒张反应降低，而CCK 8可逆转LPS的上述作用。上述结果提示CCK 8可缓解ES时的PAP升高，这可能与其调节肺动脉张力改变有关。

关键词： 胆囊收缩素; 内毒素休克; 肺动脉; 血管张力

Effects of cholecystokinirr octapeptide on the tension of pd monary artery in rabbits with endotoxic shock

DUAN Guo- Chen, LING Yi- Ling, GU Zheng Yong, WEI Peng, NIU Zhi- Yun, Yang Shifang

Department of Pathophysiology，Hebei Medical University，Shijiazhuang 050017

Abstract

For investigation of the regulatory mechanism of cholecystokinixr octapeptide(CCK 8)on pulmonary circulation in rabbits with endotoxic shock(ES) induced by lipopolysaccharides(LPS)，mean arterial pressure(MAP) and pulmonary arterial pressure(PAP) were evaluated for 5 h in five groups of rabbits:group of LPS(8 mg/ kg，i. v.)一induced ES，group of CCK 8 pretreatment(15 ug/ kg，i. v.)15min before LPS administration(8 mg/ kg，i. v.)，group of proglumide pretreatment(1 mg/ kg，i. v.)15min before LPS administration(8 mg/ kg，i. v.)，group of CCK(15 ug/ kg，i. v.)only，and normal saline(control) group .The pulmonary arterial tension was measured with isolated vascular ring tech-
nique·The results showed that LPS-induced pulmonary arterial hypertension was abolished by CCK 8.In contrast，proglu mide，a nonspecific antagonist of CCK 8 receptor，potentiated the deleterious effect of LPS .The contractile response of isolated pulmonary artery to a adrenoceptor agonist phenylephrine(PE)
was enhanced and the relaxation response to acetylcholine(ACh) was depressed significantly after LPS was injected，but the effect could be reversed by CCK 8·These results suggest that pulmonary circulation is improved by CCK 8 in ES，and the regulatory effects of CCK 8 may be brought about by modulating the pulmonary arterial tension.

Key words: cholecystokinixr octapeptide;Endotoxic shock;Pulmonary artery;vascular tension

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引用本文：

段国辰, 凌亦凌, 谷振勇, 韦鹏, 牛志云, 杨世方. 八肽胆囊收缩素对家兔内毒素休克时肺动脉张力的影响[J]. 生理学报 2003; 55 (2): .

DUAN Guo- Chen, LING Yi- Ling, GU Zheng Yong, WEI Peng, NIU Zhi- Yun, Yang Shifang. Effects of cholecystokinirr octapeptide on the tension of pd monary artery in rabbits with endotoxic shock. Acta Physiol Sin 2003; 55 (2): (in Chinese with English abstract).