过刊浏览

动脉压力感受性反射受损后血浆和心脏、肾脏组织Ang Ⅱ含量的变化

单#zheng##zheng#, 戴生明, 方凤, 苏定冯

第二军医大学长海医院儿科，基础部药理学教研室，上海200433

摘要

研究表明，动脉压力感受性反射(AB R)功能下降在高血压靶器官损伤中起独立作用。为进一步研究AB R功能下降致器官损伤的可能机制，实验采用去窦弓神经(SAD)大鼠作为AB R受损的动物模型，分别测定清醒、自由活动状态下SAD及对照的假手术组大鼠24 h动脉血压、心率、血压波动性(BP V)及心率波动性(HRV) o并采用放免法测定血浆、心脏和肾脏组织的血管紧张素11 (AngⅡ)含量。结果发现，SAD术后1周大鼠的24 h平均收缩压(SBP)、舒张压(DBP)均显著高于对照组及术后18周的慢性期SAD大鼠。SAD术后18周，24 h平均SBP .DBP及HR与假手术对照组均无显著差异;24 h收缩压波动性(SBP V)和舒张压波动性(DBP V)均显著高于对照组大鼠。SAD大鼠术后1周的血浆、心脏和肾脏组织的Ang Ⅱ含量及术后18周的血浆Ang Ⅱ水平与对照组之间相比无显著差异。而在术后慢性期(18周)，SAD大鼠的心肌及肾组织Ang Ⅱ含量显著高于假手术对照组大鼠。在术后18周时，接受慢性应激刺激的SAD大鼠，其血浆、心肌及肾组织中Ang 11水平显著高于同处应激状态下的假手术对照组大鼠及未接受应激刺激的SAD大鼠。这些结果表明，SAD术后急性期血压增高，但在慢性期平均血压并无增高，仅BP V增高;慢性期心、‘肾组织内Ang Ⅱ的分泌增加;在慢性期接受应激可致Ang Ⅱ过度分泌。上述结果提示，BPV增高和心、肾组织Ang Ⅱ含量升高与SAD大鼠发生心脏、 肾脏等器官损害有关。

关键词： 动脉压力感受性反射; 血压波动性; 血压; 血管紧张素Ⅱ; 大鼠

AngiotensinⅡcornternts in plasma，and cardiac and renal tissues of sinoaortic denervated rats

SHAN Zheng Zheng, DAI Sheng Ming, FANG Feng, SU Ding Feng

Department of Pediatrics, Changhai Hospital ； Department of Pharmacology, Faculty of Basic Medicine Second Militory Medical University Shanghai 200433

Abstract

Our previous data demonstrate that impairment of arterial baroreceptor reflex(AB助plays an independent role in hypertension target organ damage .To elucidate the mechanisms responsible for the dysfunction of ABR associated organ damage，sinoaortic denervated(SAD) rats were used as an animal model of ABR dysfunction .Twentyrfourhour continuous blood pressure(SBP and DBP)，blood
pressure variability(BPV)，heart rate(H助and H R variability(HRV)were measured in conscious and unrestrained rats .Angiotensin Ⅱ(Ang Ⅱ)in plas ma，heart and kidney was assayed by raio-i m munological assay(RIA) 1 or 18 weeks after denervation.In short-term SAD rats，twenty-four hour mean SBP and DBP increased compared with that of sham-operated rats and long-term SAD rats.No significant difference in SBP，DBP or HR was found between long-term SAD rats and sham-operated ones .Compared with the sham-operated rats，long-term SAD rats had elevated BP V.No significant change in Ang 1I levels of caridiac and renal tissues was found in short-term SAD rats .In long-term SAD rats，Ang Ⅱ level of plasma was not increased while the AngⅡ content in the heart and kidney increased .Ang Ⅱ contents of plasma and tissues in long-term SAD rats exposed to chronic stress were higher than those in the control
rats .These results show(1)in short-term SAD rats blood pressure increased，while in long-term SAD rats 24 h mean blood pressure did not increase，although BPV elevated in long-term SAD rats;(2) in long-term SAD rats，secretion of Ang Ⅱ in cardiac and renal tissues was enhanced and more Ang Ⅱreleased when the animals were exposed to chronic stress.These results suggest that elevated BP V and secretion of Ang Ⅱ may be related to the development of organ damage induced by ABR dysfunction.

Key words: arterial baroreceptor reflex;blood pressure variability;Blood pressure;Angiotensin Ⅱ;rats

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

单#zheng##zheng#, 戴生明, 方凤, 苏定冯. 动脉压力感受性反射受损后血浆和心脏、肾脏组织Ang Ⅱ含量的变化[J]. 生理学报 2003; 55 (1): .

SHAN Zheng Zheng, DAI Sheng Ming, FANG Feng, SU Ding Feng. AngiotensinⅡcornternts in plasma，and cardiac and renal tissues of sinoaortic denervated rats. Acta Physiol Sin 2003; 55 (1): (in Chinese with English abstract).