过刊浏览

一种用于吗啡心理渴求研究的自由活动动物神经元胞外记录法

刘素青, 吴晶, 杨建珍, 田少华, 陈南晖, 雷衍林, 彭沿平, 王建红, 马原野

中科院昆明动物研究所灵长类认知实验室.云南,昆明 650223

摘要

该文描述了一种将环境线索相关的大鼠吗啡成瘾模型与一种适用于自由活动动物的神经元单位放电胞外记录法相结合的实验方法。该方法成功地用于研究成瘾大鼠海马CAl区神经元的放电特征,从而在动物行为的基础上寻找到更为准确、客观的细胞学特征,并试图将这种细胞学特征作为成瘾动物特有的电生理学指标之一。

关键词： 单位放电; 自由活动大鼠; 线索相关; 吗啡戒断; 海马

Methods for single unit recording in behavioring morphine craving rat

Liu Suqing, Wu Jing, Yang Jianzhen, Tian Shaohua, Chen Nanhui, Lei Yanlin, Peng Yanping, Wang Jianhong, Ma Yuanye

Section of Primates Cognitive Brain Research,Kunming Institute of Zoology,the Chinese Academy of Sciences.Kunming 650223,Yunnan

Abstract

In this paper, one method was introduced, which was a combination of the cue-related morphine addiction model and a technique for obtaining chronic extracellular recordings of single units in freely moving rats. With the combination and improvement of this technique, We have successfully applied this new method to study the neuronal activity of the hippocampus CA1 region in morphine withdrawal rats. In all, we found some more accurate and objective cellular characteristics of hippocampal neurons, and considered these characteristics as one of electrophysiological indexes of morphine addiction rats.

Key words: Single unit recording;Freely moving rat;cue-related;Morphine withdrawal;Hippocampus

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

刘素青, 吴晶, 杨建珍, 田少华, 陈南晖, 雷衍林, 彭沿平, 王建红, 马原野. 一种用于吗啡心理渴求研究的自由活动动物神经元胞外记录法[J]. 生理学报 2004; 56 (6): .

Liu Suqing, Wu Jing, Yang Jianzhen, Tian Shaohua, Chen Nanhui, Lei Yanlin, Peng Yanping, Wang Jianhong, Ma Yuanye. Methods for single unit recording in behavioring morphine craving rat. Acta Physiol Sin 2004; 56 (6): (in Chinese with English abstract).