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白藜芦醇对离体豚鼠乳头肌的电生理效应

赵娟, 马会杰, 董京辉, 张利萍, 刘华磊, 王庆山

河北医科大学基础医学研究所生理室.河北,石家庄 050017

摘要

该文旨在应用标准玻璃微电极技术,观察白藜芦醇对离体豚鼠乳头肌的电生理效应。结果显示:(1)白藜芦醇(30,60,120 #mu#mol/L)可剂量依赖性地缩短乳头肌细胞的动作电位时程;(2)对部分去极化的乳头肌,白藜芦醇(60 #mu#mol/L)不仅缩短动作电位时程,而且降低动作电位的幅值和超射值,减慢零期最大上升速度;(3)用无钙K--H液灌流标本可完全取消RES对乳头肌细胞的作用;(4)钾通道开放剂四乙基氯化铵(TEA,20mmol/L),不能阻断白藜芦醇的电生理效应;(5)预先应用一氧化氮合酶抑制剂{sl L}--NAME(1 mmol/L),对白藜芦醇的上述效应无影响。以上结果表明,白藜芦醇可缩短正常乳头肌细胞动作电位时程,这一效应可能与其抑制钙离子内流有关,但此作用机制中NO的作用并不显著。

关键词： 电生理; 白藜芦醇; 动作电位; 乳头肌; 钙电流

Electrophysiological effects of resveratrol on guinea pig papillary muscles

Zhao Juan, Ma Huijie, Dong Jinghui, Zhang Liping, Liu Hualei, Wang Qingshan

Department of Physiology, Institute of Basic Medicine, Hebei Medical University.Shijiazhuang 050017,Hebei

Abstract

The purpose of this study was to investigate the electrophysiological effects of resveratrol on guinea pig papillary muscles and the underlying mechanism. Action potentials were recorded using intracellular microelectrode technique. The results obtained are as follows: (1) In normal papillary muscles, resveratrol (30, 60, and 120 #mu#mol/L) shortened the duration of action potential (APD) in a concentration-dependent manner; (2) In partially depolarized papillary muscles, resveratrol (60 #mu#mol/L) not only shortened APD, but also decreased the amplitude of action potential (APA), overshoot (OS) and maximal rate of depolarization in phase 0 (V_(max)); (3) Perfusion with Ca~(2+)-free K-H solution, completely abolished the effects of resveratrol (60 #mu#mol/L) on papillary muscles; (4) Application of potassium channel blocker tetraethylammonium chloride (TEA, 20mmol/L) did not prevent the effect of resveratrol (60 祄ol/L) on action potential; (5) Pretreatment with N~(G)-nitro-L-arginine methyl ester (L-NAME, 1 mmol/L), a nitric oxide (NO) synthase inhibitor, failed to abolish the effect of resveratrol (60 #mu#mol/L). All these results indicate that the electrophysiological effects of resveratrol on guinea pig papillary muscles are likely due to the reduction in calcium influx, which might not be mediated by NO.

Key words: electrophysiology;resveratrol;Action potential;Papillary muscle;calcium current

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引用本文：

赵娟, 马会杰, 董京辉, 张利萍, 刘华磊, 王庆山. 白藜芦醇对离体豚鼠乳头肌的电生理效应[J]. 生理学报 2004; 56 (6): .

Zhao Juan, Ma Huijie, Dong Jinghui, Zhang Liping, Liu Hualei, Wang Qingshan. Electrophysiological effects of resveratrol on guinea pig papillary muscles. Acta Physiol Sin 2004; 56 (6): (in Chinese with English abstract).