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表皮生长因子对培养的气道上皮细胞抗臭氧损伤的保护作用

秦晓群, 孙秀泓, 罗自强, 管茶香, 张长青

(湖南医科大学生理学教研室，长沙410018)

摘要

研究观察到臭氧(03)对体外培养经3H-UdR标记的兔气道上皮细胞有明显细胞毒性作用，且损伤程度与03作用时间呈正相关。03暴露组细胞内丙二醛(MDA)产生增多((P< 0. 01)，提示03损伤细胞的机制与胞膜脂质过氧化有关。表皮生长因子(EGF)可明显降低。:所梦约3H释放率(P<0. 01)、降低0:的细胞毒指数及细胞内MDA含量(P<0. O1),证明EGF对弋道上皮细胞有保护作用。进一步还观察到浓度为5 ng/ml的EGF可以取消。:所引起的细胞内还原型谷胱甘肽(GSH)含量降低((P<0. 01)，并增加细胞内谷胱甘肽总含量(P<0. 05)，但不能改变03所致的氧化型谷胧甘肤(GSSG)含量的增加(P> 0. 05)，对GSH/GSSG比值也无明显提高，这些都提示EGF的细胞保护机理可能与其促进细胞内谷胱甘肽合成有关，而对GSSG转化为GSH的还原过程影响不明显。

关键词： 气道上皮细胞; 臭氧; 表皮生长因子; 细胞保护; 谷胱甘肽; 丙二醛

CYTOPROTECTIVE EFFECT OF EPIDERMAL GROWTH FACTOR ON CULTURED RABBIT AIRWAY EPITHELIAL CELLS EXPOSED TO OZONE

QIN XIAO-QUN, SUN XIU-HONG, LOU ZI-QIANG , GUAN CHA-XIANG , ZHANG CHANG-QING

(Department of Physiology,Human Medical University, Changsha 410078)

Abstract

In this study，it was observed that ozone (03) exposure has cytotoxic effects on cultured airway epithelial cells，which was positively related with exposure duration. Both the production of malondialdehyde (MDA) and the 3H release in the exposure group were much higher than the control (P<0. 01)，suggesting that lipoperoxidation occurring in the cell membrane was reponsible for the cellular injury observed under 03 attack. EGF at low concentration (5 ng/ml) showed cytoprotective effect on airway epithelial cells exposed to 03 as shown' by attenuated MDA production and decrease of 3H release and cytotoxic index (P<0. 0l).Pretreatment with EGF could reverse the depletion of intracellular GSH content as a result of 03 exposure and increase the total glu
tathionein the cells. The above results suggest that the cytoprotection of EGF on airway epithelial cells may be related to its promotive effect on GSH synthesis.

Key words: airway epithelial cells;ozone;;glutathione; malondialdehyde ;

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引用本文：

秦晓群, 孙秀泓, 罗自强, 管茶香, 张长青. 表皮生长因子对培养的气道上皮细胞抗臭氧损伤的保护作用[J]. 生理学报 1996; 48 (2): .

QIN XIAO-QUN, SUN XIU-HONG, LOU ZI-QIANG , GUAN CHA-XIANG , ZHANG CHANG-QING. CYTOPROTECTIVE EFFECT OF EPIDERMAL GROWTH FACTOR ON CULTURED RABBIT AIRWAY EPITHELIAL CELLS EXPOSED TO OZONE . Acta Physiol Sin 1996; 48 (2): (in Chinese with English abstract).