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刺激面神经核对前包钦格复合体呼吸神经元放电活动的影响及其递质机制

严亨秀, 张承武, 郑煜

四川大学华西基础医学与法医学院生理教研室.四川,成都 610041

摘要

实验选用健康成年SD大鼠,观察电刺激面神经核对前包钦格复合体(pre--Bötzinger complex,PBC)呼吸神经元(RNs)放电活动的影响,并观察微电泳6--氰基--7--硝基喹喔啉--2,3--二酮(CNQX)、荷包牡丹碱(BIC)、士的宁(Stry)和阿托品(Atr)对电刺激面神经核引起的PBC RNs放电变化的拮抗效应,以进一步探讨面神经核是否参与呼吸调节及其可能的神经机制。在12只面运动神经元逆行溃变大鼠同侧PBC内共记录到各类RNs116个,电刺激溃变侧面神经核时,前吸气(Pre--I)神经元(24/26个)和吸气(I)神经元(30/35个)主要表现为兴奋,呼气(E)神经元(20/22个)和吸气--呼气(I--E)跨时相神经元(28/33个)表现为抑制。CNQX可完全或部分拮抗电刺激面神经核对Pre--I(18/24)和I(23/27)神经元的兴奋效应; Stry可拮抗电刺激面神经核对Pre--I(12/18)和I(14/23)神经元的瞬时抑制效应以及对I--E(20/28)和E(9/16)神经元的抑制效应;BIC可拮抗电刺激面神经核对I--E(22/25)和E(9/9)神经元的抑制效应;微电泳Atr对各类RNs的放电变化无明显作用。这些结果表明,面神经核非运动神经元可能通过向PBC的纤维投射,以Glu、GABA和Gly为神经递质或调质,调节PBC RNs的活动,进而参与对呼吸运动的调节。

关键词： 面神经核; 非运动神经元; 前包钦格复合体; 呼吸神经元; 放电; 递质

Effect of stimulation of the facial nucleus on discharge of respiratory neurons in the pre--Bötzinger complex and its neurotransmitter mechanism in rats

Yan Hengxiu, Zhang Chengwu, Zheng Yu

Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University.Chengdu 610041,Sichuan

Abstract

The experiments were carried out on adult Sprague-Dawley rats. We investigated the discharge response of respiratory neurons (RNs) in the pre-Bötzinger complex (PBC) to electrical stimulation of the facial nucleus in which the motor neurons were retrtogradely degenerated and the antagonistic effects of microiontophoresis of CNQX, bicuculline (BIC), strychnine (Stry) and atropine on the discharge responses of the neurons. In 12 rats with retrograde degeneration of the facial motor neurons, 116 RNs in the PBC ipsilateral to the facial nerve sectioned were extracellularly recorded. The response of pre-inspiratory (Pre-I) (24/26) and inspiratory (I) (30/35) neurons to the electrical stimulation of the facial nucleus was mainly excitatory, and the response of expiratory (E) (20/22) and inspiratory-expiratory phase-spanning (I-E) (28/33) neurons was mainly inhibitory. CNQX partially or completely block the excitatory effect of the stimulation on Pre–I (18/24) and I (23/27) neurons. Stry could partially or completely block the immediate transient inhibition on Pre–I (12/18) and I (14/23) neurons and the inhibitory effect on I-E (20/28) and E (9/16) neurons induced by the stimulation. BIC partially or completely blocked the inhibitory effect of I-E (22/25) and E (9/9) neurons induced by the stimulation. Atropine did not have obvious influence on the response of RNs to the stimulation. These results suggest that non-motoneurons in the facial nucleus may participate in the modulation of respiration by affecting the activities of RNs in the PBC and Glu, GABA and Gly could serve as the neurotransmitters or modulators to regulate the activities of the RNs in the PBC and hence the rhythmic respiratory movement.

Key words: Facial nucleus;Non-motoneurons;Pre-Botzinger complex;Respiratory neurons;Discharge;Neurotransmitters

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引用本文：

严亨秀, 张承武, 郑煜. 刺激面神经核对前包钦格复合体呼吸神经元放电活动的影响及其递质机制[J]. 生理学报 2004; 56 (6): .

Yan Hengxiu, Zhang Chengwu, Zheng Yu. Effect of stimulation of the facial nucleus on discharge of respiratory neurons in the pre--Bötzinger complex and its neurotransmitter mechanism in rats. Acta Physiol Sin 2004; 56 (6): (in Chinese with English abstract).