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血管活性肠肽、降钙素基因相关肽及其受体在气道高反应动物肺内的时空分布

任雁宏, 秦晓群, 管茶香, 罗自强, 张长青, 孙秀泓

中南大学湘雅医学院生理学教研室.湖南,长沙 410078

摘要

为探讨内源性神经肽在气道高反应性形成中的作用,以臭氧应激损伤动物气道上皮细胞,建立气道高反应性动物模型,并观察臭氧应激不同时间肺内血管活性肠肽(VIP)、降钙素基因相关肽(CGRP)含量变化以及VIP受体(VIPR1)、CGRP受体(CGRP1)mRNA在肺内表达、分布的改变。实验观察到,臭氧应激组动物吸入乙酰甲胆碱后气道阻力高于正常对照组,肺内呈现明显的炎症改变。随臭氧应激时间延长,肺组织匀浆中VIP、CGRP浓度呈先增高后降低的双向改变,CGRP达峰值时间早于VIP。VIPR1、CGRPR1 mRNA表达亦经历了双向过程,VIPR1峰值持续时间长于CGRPR1。在无应激对照组动物,肺间质、支气管上皮细胞、血管内皮细胞、平滑肌细胞均有VIPR1、CGRPR1 mRNA表达。随应激时间的延长,阳性细胞呈斑片状集中于气管、血管周围,染色强度增加,至臭氧应激d8,阳性染色细胞减少。因此推测,臭氧应激可以诱导动物气道高反应性的形成。在炎症的早期,以CGRP的作用为主,与肺损伤早期炎症信号的传递有关,以清除刺激原、及时终止致病原的作用;炎症后期以保护机体、促进修复、减轻损伤为主,VIP发挥主要作用。

Temporal and spatial distribution of VIP, CGRP and their receptors in the development of airway hyperresponsiveness in the lungs

Ren Yanhong, Qin Xiaoqun, Guan Chaxiang, Luo Ziqiang, Zhang Changqing, Sun Xiuhong

Department of Physiology,Xiangya Medical School,Central South University.Changsha 410078,Hunan

Abstract

From the results, it is concluded that ozone-stressing can induce the development of AHR, in which VIP and CGRP may play important roles. That implies, through binding to CGRPR1, CGRP stimulates an early inflammation response which contributes in cleaning up of irritants, while VIP exerts a later dampening of pulmonary inflammation response. These two neuropeptides may play sequential and complementary roles in the development of AHR.

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引用本文：

任雁宏, 秦晓群, 管茶香, 罗自强, 张长青, 孙秀泓. 血管活性肠肽、降钙素基因相关肽及其受体在气道高反应动物肺内的时空分布[J]. 生理学报 2004; 56 (2): .

Ren Yanhong, Qin Xiaoqun, Guan Chaxiang, Luo Ziqiang, Zhang Changqing, Sun Xiuhong. Temporal and spatial distribution of VIP, CGRP and their receptors in the development of airway hyperresponsiveness in the lungs. Acta Physiol Sin 2004; 56 (2): (in Chinese with English abstract).