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电针和碱性成纤维生长因子(bFGF)对脑缺血时神经细胞的保护作用

李荣, 郭景春, 程介士

复旦大学上海医学院针刺原理研究所.上海(200032)

摘要

采用暂时性脑缺血再灌汪大鼠模型，及H&E、TUNEL细胞染色等实验技术，观察电针或碱性成纤维生长因子，以及两者合用对缺血性神经细胞死亡的影响。实验结果表明，电针与碱性成纤维生长因子合用与单纯使用电针或碱性成纤维生长因子相比，可明显减少暂时性脑缺血再灌注后神经细胞坏死和凋亡。提示碱性成纤维生长因子与电针可具有互补或加强的神经保护作用。两者合用具有一定的临床实际价值。

关键词： 脑缺血; 电针; 碱性成纤维生长因子; 神经保护作用

The protective effect of basic fibroblast growth factor (bFGF) and electroacupuncture on neurons against cerebral ischemia

Li Rong, Guo Jingchun, Cheng Jieshi

National Key laboratory of Medical Naurobiology, Institute of Acupuncture Research, Shanghai Medical College, Fudan University.Shanghai(200032)

Abstract

Transient cerebral ischemia/reperfusion rat model was adopted, and the method of H&E staining, TUNEL staining (TdT-mediated dUTP Nick End Labeling) were used to observe the effect of electroacupuncture (EA) and basic fibroblast growth factor (bFGF) on neuronal death. The result evinced that the combination of EA and bFGF could evidently reduce the neuronal death, including both necrosis and apoptosis, compared with EA or bFGF application alone. It is suggested that bFGF and EA can complement each other and enhance the protective effect following cerebral ischemia.

Key words: Cerebral ischemia/reperfusion;Electroacupuncture(EA);Basic fibroblast growth factor (bFGF);Neuro-protective effect

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引用本文：

李荣, 郭景春, 程介士. 电针和碱性成纤维生长因子(bFGF)对脑缺血时神经细胞的保护作用[J]. 生理学报 2002; 54 (4): .

Li Rong, Guo Jingchun, Cheng Jieshi. The protective effect of basic fibroblast growth factor (bFGF) and electroacupuncture on neurons against cerebral ischemia. Acta Physiol Sin 2002; 54 (4): (in Chinese with English abstract).