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肾血管性高血压大鼠发病过程中心血管AT_1－受体自身抗体的变化

支建明, 赵录英, 焦向英, 赵荣瑞

山西医科大学心血管生理研究室,太原(030001)

摘要

实验观察了肾血管性高血压（RVH）大鼠血浆中抗AT_1－受体自身抗体在发病过程中的作用及其变化规律。采用两肾一夹Goldblatt RVH模型，以合成的大鼠AT_1－受体细胞外第二环165－191位氨基酸列作为特异性抗原，用SA－ELISA法检测大鼠血清中抗AT_1－受体自身抗体。结果表明，RVH模型组术后2周时大鼠血清中抗AT_1－受体自身抗体的阳性率和平均滴度与术前相比明显增高，较高的阳性率和平均滴度持续几周后逐渐下降，12周时下降到正常水平。结果提示，自身免疫机制参与了高血压的形成，抗AT_1－受体自身抗体可能与心肌肥厚有关。

关键词： 肾血管性高血压; 血管紧张素Ⅱ; 受体; 自身抗体

Changes in autoantibody against cardiovascular AT_1-receptor during development of renovascular hypertension in rats

Zhi Jianming, Zhao Luying, Jiao Xiangying, Zhao Rongrui

Department of Physiology, Shanxi Medical University,Taiyuan(030001)

Abstract

The aim of this study was to observe the change in angiotensin II receptor subtype 1 (AT_1) autoantibody during the development of renovascular hypertension (RVH). The Goldblatt renovascular hypertension model was established by the two-kidney one-clip method, and a synthetic peptide corresponding to amino acid sequence 165-191 of the second extracellular loop of the AT_1-receptor was used as the antigen. Sera AT_1-receptor autoantibody was detected by SA-ELISA. It was shown that two weeks after operation both the frequency of occurrence and the titre of autoantibodies to AT_1-receptor were significantly increased as compared with the pre-treatment control. The increase in autoantibodies lasted several weeks and then decreased gradually to the pre-clipping level at 12 weeks. It is suggested that autoimmune mechanisms are involved in the pathogenesis of renovascular hypertension and the AT_1 autoantibodies may be one of the mechanisms leading to cardiac hypertrophy.

Key words: Renovascular hypertension;Angiotensin-II;Receptor;Autoantibody

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引用本文：

支建明, 赵录英, 焦向英, 赵荣瑞. 肾血管性高血压大鼠发病过程中心血管AT_1－受体自身抗体的变化[J]. 生理学报 2002; 54 (4): .

Zhi Jianming, Zhao Luying, Jiao Xiangying, Zhao Rongrui. Changes in autoantibody against cardiovascular AT_1-receptor during development of renovascular hypertension in rats. Acta Physiol Sin 2002; 54 (4): (in Chinese with English abstract).