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Endogenous carbon monoxide attenuates lung injury following ischemia-reperfusion in the hind limbs of rats

Zhou Junlin, Ling Yiling, Jin Guohua, Zhang Junlan, Shi Zhongli, Huang Xinli

Department of Pathophysiology, Hebei Medical University,Shijiazhuang(050017)

摘要

To investigate the role of endogenous heme oxygenase (HO)/carbon monoxide (CO) system in the lung injury as assessed by lung histology, polymorphonuclear count, malondialdehyde content and wet-to-dry weight ratio following ischemia-reperfusion (I/R) of hind limbs, zinc protoporphyrin (ZnPP), an inhibitor of HO activity, was used, and the lung HO activity and blood carboxyhemoglobin (COHb) level were measured. The results showed that HO activity and COHb level were increase significantly and lung injury occurred after limb I/R. After administration of ZnPP, the lung injury was further aggravated while the HO activity and COHb level were significantly decreased. These findings suggest that up-regulation of HO activity followed by subsequent CO production attenuates the lung injury induced by limb I/R in rats.

关键词： 血红素氧化酶; 一氧化碳; 再灌注损伤; 肢体; 肺

内源性一氧化碳减轻大鼠双侧后肢缺血再灌注所致的肺损伤

周君琳, 凌亦凌, 金国华, 张君岚, 史中立, 黄新莉

河北医科大学病理生理学教研室,石家庄(050017)

Abstract

通过观察血红素氧化酶（HO）阻断剂-锌原卟啉（ZnPP）对肺组织、肺泡间质多形核白细胞数目、肺组织丙二醛含量和湿重干重之比的影响，并对肺组织HO活性和血内碳氧血红蛋白水平（COHb）进行检测，以探讨内源性HO/一氧化碳（CO）在肢体缺血再灌注（I/R）所致肺损伤中的作用。结果发现，大鼠双侧后肢I/R可导致急性肺损伤，同时使肺组织中HO活性和血内COHb水平显著升高；应用ZnPP预处理可使HO活性和内源性CO生成增多可减轻大鼠肢体I/R所致的肺损伤。

Key words: heme oxygenase;Carbon monoxide;Reperfusion injury;Extre mities;Lung

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引用本文：

Zhou Junlin, Ling Yiling, Jin Guohua, Zhang Junlan, Shi Zhongli, Huang Xinli. Endogenous carbon monoxide attenuates lung injury following ischemia-reperfusion in the hind limbs of rats[J]. 生理学报 2002; 54 (3): .

周君琳, 凌亦凌, 金国华, 张君岚, 史中立, 黄新莉. 内源性一氧化碳减轻大鼠双侧后肢缺血再灌注所致的肺损伤. Acta Physiol Sin 2002; 54 (3): (in Chinese with English abstract).