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缺氧对大鼠大脑皮质细胞色素氧化酶亚基Ⅰ、Ⅳ表达协同性的影响

谭小玲, 柳君泽, 曹利飞, 邓忠才, 李英和

第三军医大学高原军事医学系病理生理学与高原生理学教研室,全军高原生理和高原病研究重点实验室.重庆 400038

摘要

缺氧2、5d,COXⅠmRNA增加,缺氧15、30d时下降至对照水平。缺氧2、5和15d时,COXⅣmRNA显著增加,缺氧30d时降低,与对照组差异非常显著。COXⅣ、ⅠmRNA比值在缺氧15d时最高,其它各缺氧组与对照组的差异无显著意义。各组COXⅠ、Ⅳ蛋白量及其比值均无显著差异。上述结果表明,缺氧可影响COXⅠ、ⅣmRNA的表达及其协同性,但对蛋白的表达及其协同性没有显著影响,提示转录后调节是缺氧过程中线粒体内COXⅠ、Ⅳ蛋白表达协同的主要机制。

关键词： 细胞色素氧化酶; 基因表达; 大脑皮质; 大鼠; 缺氧

Effects of hypoxic exposure on coordinative expression of cytochrome oxidase subunits Ⅰ and Ⅳ in rat cerebral cortex

Tan Xiaoling, Liu Junze, Cao Lifei, Deng Zhongcai, Li Yinghe

Department of Pathophysiology,College of High Altitude Military Medicine,the Third Military Medical University.Chongqing 400038

Abstract

The results showed that COX I mRNA increased significantly at 2 and 5 d, and decreased to the control level at 15 and 30 d; COX IV mRNA remarkably increased at 2, 5 and 15 d, and dropped below the control level at 30 d. The mRNA ratio of COX IV to COX I reached a peak at 15 d, but showed no differences between other time points. The Western blot analysis of COX I and COX IV in isolated at cerebral cortex mitochondria showed no obvious changes during hypoxic exposure. Our findings demonstrate that hypoxia can affect mRNA expression of COX I and COX IV and their coordination, while protein expression of both subunits are stable and coordinative. This study suggests that the expression of COX I and COX IV proteins during hypoxic exposure is coordinately regulated by post-transcriptional mechanisms.

Key words: Cytochrome oxidase;Gene expression;Cerebral cortex;Rat;Hypoxia

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引用本文：

谭小玲, 柳君泽, 曹利飞, 邓忠才, 李英和. 缺氧对大鼠大脑皮质细胞色素氧化酶亚基Ⅰ、Ⅳ表达协同性的影响[J]. 生理学报 2002; 54 (6): .

Tan Xiaoling, Liu Junze, Cao Lifei, Deng Zhongcai, Li Yinghe. Effects of hypoxic exposure on coordinative expression of cytochrome oxidase subunits Ⅰ and Ⅳ in rat cerebral cortex. Acta Physiol Sin 2002; 54 (6): (in Chinese with English abstract).