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兔精子发生过程中cyclin B1基因表达和蛋白定位的发育阶段依赖性

孔维华, 颜山, 顾正, 左嘉客

山东大学生命科学学院.山东,济南 250100；上海市计划生育科学研究所计划生育药具国家重点实验室.上海 200032

摘要

利用原位杂交和免疫组化等方法,研究兔精子发生过程中生精细胞cyclin B1 mRNA的表达和蛋白定位特点。结果显示:兔生精上皮中cyclin B1 mRNA主要分布在初级精母细胞中,直至圆形精子细胞仍然存在,于精子细胞的变态过程中逐渐消失。在伸长的精子细胞和精子中未检测出cyclin B1 mRNA。Cycln B1蛋白在进入分裂期的精原细胞和精母细胞中表达;在圆形精子细胞和伸长的精子细胞中呈现大量的cyclin B1蛋白。上述结果表明:在兔精子发生过程中,cyclin B1 mRNA表达和蛋白定位具有发育阶段依赖性的特征。

关键词： cyclin B1; 兔; 精子发生; 生精细胞发育阶段依赖性

Developmental stage-dependence of cyclin B1 protein localization and gene expression in rabbit spermatogenesis

Kong Weihua, Yan Shan, Gu Zheng, Zuo Jiake

College of Life Science,Shandong University.Jinan 250100,Shandong；China

Abstract

By means of in situ hybridization and immunohistochemistry, the protein localization and gene expression of cyclin B1 in spermatogenic cells were characterized during the spermatogenesis of rabbits. The results showed that the cyclin B1 mRNA in rabbit spermatogenic epithelium was expressed dominantly in primary spermatocytes. The expression was observed in round spermatids with a gradual decline in the process of metamorphosis of the spermatids, but not in elongated spermatids and sperms. Cyclin B1 protein was expressed in mitotic spermatogonia and meiotic spermatocytes and was observed predominantly in round and elongated spermatids. These results indicate that the expression of cyclin B1 mRNA and localization of cyclin B1 protein are dependent on the developmental stages of spermatogenic cells.

Key words: Cyclin Bl;Rabbit;Spermatogenesis;Developmental stage-dependence of spermatogenic cells

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引用本文：

孔维华, 颜山, 顾正, 左嘉客. 兔精子发生过程中cyclin B1基因表达和蛋白定位的发育阶段依赖性[J]. 生理学报 2002; 54 (5): .

Kong Weihua, Yan Shan, Gu Zheng, Zuo Jiake. Developmental stage-dependence of cyclin B1 protein localization and gene expression in rabbit spermatogenesis. Acta Physiol Sin 2002; 54 (5): (in Chinese with English abstract).