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用荧光标记钠钾ATP酶显示气道感受器结构

王玉芳, 於峻

路易斯维尔大学内科系与生理系.美国,肯塔基州 40292

摘要

阐述了一种新颖方法,利用共聚焦显微镜并结合免疫组织化学方法,采用Na~(+)/K~(+)-ATPase作为标记,对家兔呼吸道感受器进行了观察。经过处理的气道组织结构,背景清晰,感受器部位着色强。观察到的整个感受器,结构复杂,呈树枝状,感受器末梢膨大,形成叶片状。首次以高清晰度展示了呼吸气道中单个感受器的整体结构,改变了需要以手绘来刻画感受器的现状。无疑,这种新方法能促进了解感受器的结构及其生理活动的机理。

关键词： 气道感受器; 感受神经; 钠泵; 免疫组化

Na~(+)/K~(+)-ATPase as a marker for detecting pulmonary sensory receptors

Wang Yufang, Jerry Yu

Department of Medicine,and Physiology and Biophysics,University of Louisville.Louisville, KY 40292

Abstract

Here we describe a new approach using confocal microscopy to examine the structure of immunohistochemically stained airway receptors by using Na~(+)/K~(+)-ATPase as a marker. This method permits detailed observation of pulmonary receptor structures and their relationship to target tissue in the rabbit. The parent axon has multiple branches that supply the receptor structure. Each structure has multiple endings that form leaf-like terminals. This technique permits examining pulmonary receptor structures in detail, and should assist in identifying the morphology of the receptors and the mechanisms of receptor activation.

Key words: Airway receptor;Sensory nerve;Sodium pump;Immunohistoche mistry

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引用本文：

王玉芳, 於峻. 用荧光标记钠钾ATP酶显示气道感受器结构[J]. 生理学报 2002; 54 (5): .

Wang Yufang, Jerry Yu. Na~(+)/K~(+)-ATPase as a marker for detecting pulmonary sensory receptors. Acta Physiol Sin 2002; 54 (5): (in Chinese with English abstract).