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自发性高血压大鼠心肌和血管组织牛磺酸的转运障碍

石彦荣, 齐永芬, 卜定方, 高霖, 王冬彦, 蒋宏峰, 庞永正, 唐朝枢

北京大学第一医院心血管病研究所.北京 100034

摘要

在自发性高血压大鼠(SHR)的心肌和主动脉血管组织上观察牛磺酸(taurine)转运和牛磺酸转运体(TAUT)mRNA的改变。结果显示,与对照组WKY大鼠相比,SHR组血浆牛磺酸水平和牛磺酸释放量增加,而心肌和血管组织牛磺酸水平和TAUT mRNA含量均降低,牛磺酸最大转运速率(V_(max))分别低24%和35%(P<0.05),米氏常数(Km)值分别高16%和39%(P<0.05)。这些结果提示,SHR的心肌和血管组织牛磺酸转运障碍可能与TAUT活性和亲和力降低及TAUT基因水平的下调有关。

关键词： 牛磺酸; 转运体; mRNA; 自发性高血压大鼠; 心肌; 血管

Dysfunction of myocardial and vascular taurine transport in spontaneously hypertensive rats

Shi Yanrong, Qi Yongfen, Bu Dingfang, Gao Lin, Wang Dongyan, Jiang Hongfeng, Pang Yongzheng, Tang Chaoshu

Institute of Cardiovascular Disease Research,The First Hospital of Peking University.Beijing 100034

Abstract

The alterations of taurine transport and the expression of taurine transporter (TAUT) mRNA in myocardium and aortic wall were investigated in spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats. It was demonstrated that plasma taurine concentration and taurine release from myocardium and aortic wall in SHR were higher than those in WKY rats, whereas taurine content, taurine uptake and TAUT mRNA in myocardium and aortic wall of SHR were lower than those of WKY rats. In SHR, the maximal velocity (V_(max)) of taurine transportration in myocardium and aortic wall was lower by 24% (P<0.05) and 35% (P<0.05) than that in WKY, their michaelis constants (Km) values were higher by 16% (P<0.05) and 39% (P<0.05), respectively. The results suggest that there is dysfunction of taurine transport in myocardium and aortic wall in SHR, which may be partly resulted from the decrease of TAUT activity and affinity, and down-regulation of TAUT gene expression.

Key words: Taurine;Transporter;mRNA;Spontaneously hypertensive rat;Myocardial;Vessels

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引用本文：

石彦荣, 齐永芬, 卜定方, 高霖, 王冬彦, 蒋宏峰, 庞永正, 唐朝枢. 自发性高血压大鼠心肌和血管组织牛磺酸的转运障碍[J]. 生理学报 2002; 54 (5): .

Shi Yanrong, Qi Yongfen, Bu Dingfang, Gao Lin, Wang Dongyan, Jiang Hongfeng, Pang Yongzheng, Tang Chaoshu. Dysfunction of myocardial and vascular taurine transport in spontaneously hypertensive rats. Acta Physiol Sin 2002; 54 (5): (in Chinese with English abstract).