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三羟异黄酮对离体家兔股动脉张力的影响及其机制

吉恩生, 李清, 何瑞荣

河北医科大学基础医学研究所生理教研室.河北,石家庄 050017

摘要

在苯肾上腺素(PE,1#mu#mol/L)引起血管收缩的基础上,植物雌激素三羟异黄酮(GST)(10-40#mu#mol/L)剂量依赖性地舒张离体家兔股动脉;去除血管内皮显著地抑制GST引起的舒张;在内皮完整情况下,预先应用NOS抑制剂L-NAME(100#mu#mol/L)也可显著地抑制GST引起的舒张,提示GST的舒血管作用是内皮依赖的,并与一氧化氮有关;在内皮完整的和去除内皮的股动脉环,预先应用L-型钙通道激动剂Bay K8644(0.5#mu#mol/L)也显著抑制由GST引起的血管舒张。以上结果表明,GST引起的兔股动脉的舒张是部分内皮依赖的,且与拮抗钙有关。

关键词： 三羟异黄酮; 一氧化氮; 内皮功能; Bay K8644

Action of genistein on tension of isolated rabbit femoral artery and its mechanism

Ji Ensheng, Li Qing, He Ruirong

Department of Physiology,Institute of Basic Medicine,Hebei Medical University.Shijiazhuang 050017,Hebei

Abstract

The results are as follows: (1) genistein (10-40 #mu#mol/L) relaxed femoral arterial rings in a concentration-dependent manner under the condition of precontraction induced by phenylephrine (PE, 1#mu#mol/L); (2) removal of the endothelium significantly inhibited genistein-induced relaxation; (3) pretreatment with NOS inhibitor N~(G)-nitro-L-arginine methyl ester (L-NAME, 100 #mu#mol/L) also significantly inhibited this relaxation by genistein, implying that the concentration-dependent vasorelaxation caused by genistein is endothelium-dependent and involved nitric oxide; and (4) pretreatment with an L-type calcium channel agonist, Bay K 8644 (0.5 #mu#mol/L), also significantly inhibited the genistein-induced relaxation in both endothelium-intact and endothelium-denuded rings. The results suggest that the genistein-induced vascular relaxation of these rabbit arteries is partially endothelium-dependent and involves calcium antagonistic mechanism.

Key words: Genistein;Nitric oxide;Endothelial function;Bay K 8644

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引用本文：

吉恩生, 李清, 何瑞荣. 三羟异黄酮对离体家兔股动脉张力的影响及其机制[J]. 生理学报 2002; 54 (5): .

Ji Ensheng, Li Qing, He Ruirong. Action of genistein on tension of isolated rabbit femoral artery and its mechanism. Acta Physiol Sin 2002; 54 (5): (in Chinese with English abstract).