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血管紧张素Ⅱ对模拟缺血心室肌细胞L-型钙通道的影响

王文伟, 朱依纯, 姚泰, 郑平, 龚茜玲

复旦大学上海医学院生理学教研室,医学神经生物学国家重点实验室.上海 200032

摘要

实验研究了血管紧张素Ⅱ(AngⅡ)对模拟缺血心室肌细胞L-型钙离子通道的作用。用胶原酶酶解法急性分离豚鼠心室肌细胞,以全细胞膜片钳方法记录心室肌细胞的L-型钙电流(I_(Ca,L))。采用低氧、无糖、高乳酸和酸中毒综合方式模拟缺血液灌流,造成心室肌细胞的模拟缺血,并在缺血的基础上继续用含100nmol/L AngⅡ灌流细胞,观察AngⅡ对模拟缺血心室肌细胞钙离子通道的影响。实验结果显示:模拟缺血时I_(Ca,L)峰值电流明显减小,最大激活电压为0mV;AngⅡ能抵抗模拟缺血对I_(Ca,L)的抑制效应,使I_(Ca,L)峰值电流增大,并使最大激活电压左移至-10mV。

关键词： 血管紧张素Ⅱ; 心肌缺血; 全细胞膜片钳; L-型钙通道

Effect of angiotensin Ⅱ on L-type calcium channel in ischemic ventricular myocytes of the guinea pig

Wang Wenwei, Zhu Yichun, Yao Tai, Zheng Ping, Gong Qianling

Department of Physiology,Shanghai Medical College of Fudan University,National Key Laboratory for Medical Neurobiology.Shanghai 200032

Abstract

Using whole cell patch clamp, the effects of angiotensin II (Ang II) on the current L-type calcium channel (I_(Ca,L)) were observed in guinea pig isolated ventricular myocytes under simulated ischemia condition, which was realized through hypoxia, glucose deficiency, high lactic acid and acidosis.. The results showed that, under the condition of simulated ischemia, the peak of L_(Ca,L) was reduced with maximal activation potential at 0 mV. Administration of Ang II (100 nmol/L) enhanced the peak of I_(Ca,L) during ischemia and shifted the maximal activation potential to -10 mV. The possible mechanism of these effects is discussed.

Key words: Angiotensin II;Myocardial ischemia;Whole cell pacth clamp;L-type calcium channel

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引用本文：

王文伟, 朱依纯, 姚泰, 郑平, 龚茜玲. 血管紧张素Ⅱ对模拟缺血心室肌细胞L-型钙通道的影响[J]. 生理学报 2002; 54 (5): .

Wang Wenwei, Zhu Yichun, Yao Tai, Zheng Ping, Gong Qianling. Effect of angiotensin Ⅱ on L-type calcium channel in ischemic ventricular myocytes of the guinea pig. Acta Physiol Sin 2002; 54 (5): (in Chinese with English abstract).