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植物性雌激素genistein对哇巴因所致豚鼠乳头状肌迟后去极化及触发活动的效应

马韬, 何瑞荣, 王川

河北医科大学基础医学研究所生理室.河北,石家庄 050017；河北医科大学基础医学研究所药理室.河北,石家庄 050017

摘要

应用标准玻璃微电极技术,研究了植物性雌激素genistein(GST)对哇巴因所引起的豚鼠乳头状肌迟后去极化(DAD)及触发活动(TA)的效应。结果如下:(1)预先给予GST(10,50,100#mu#mol/L)剂量依赖性地抑制哇巴因(1#mu#mol/L)所引起的豚鼠乳头状肌DAD及TA;(2)预先应用一氧化氮合酶抑制剂L-NAME(1mmol/L),不影响GST(50#mu#mol/L)对DAD及TA的效应;(3)单独应用17#beta#-雌二醇(E_(2),5#mu#mol/L)或GST(10#mu#mol/L)对DAD及TA无明显影响,而联合应用相同剂量的GST和E_(2)则产生明显抑制效应。以上结果提示,GST可能通过抑制钙离子内流从而具有抗心律失常作用,这对于心血管系统的保护有一定意义。

关键词： 植物性雌激素; genistein; 迟后去极化; 乳头状肌; 电生理; 哇巴因; 17β-雌二醇

Effects of phytoestrogen genistein on delayed afterdepolarization and triggered activity induced by ouabain in guinea pig papillary muscles

Ma Tao, He Ruirong, Wang Chuan

Department of Physiology,Institute of Basic Medicine,Hebei Medical University.Shijiazhuang 050017,Hebei；China

Abstract

The results are as follows: (1) DAD and TA induced by ouabain (1 #mu#mol/L) were makedly inhibited by pretreatment with GST (10, 50, 100 #mu#mol/L) in a concentration-dependent manner. (2) NG-nitro-L-arginine methyl ester (L-NAME, 1 mmol/L), an NO synthase inhibitor, failed to affect the above effects of GST. (3) 5 #mu#mol/L 17 #beta#-estradiol (E_(2)) or 10 #mu#mol/L GST alone showed no effects on DAD and TA, whereas GST combined with E_(2) at the same doses exerted significant inhibitory effects on DAD and TA. Since GST is known to reduce the calcium influx, it is suggested that GST might have antiarrhythmic effects, possibly by reducing calcium influx. The antiarrhythmic effects of GST may contribute to its cardioprotective action.

Key words: Phytoestrogen;genistein;Delayed afterdepolarization;Papillary muscles;Electrophysiology;Ouabain;17#beta#-estradiol

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引用本文：

马韬, 何瑞荣, 王川. 植物性雌激素genistein对哇巴因所致豚鼠乳头状肌迟后去极化及触发活动的效应[J]. 生理学报 2002; 54 (5): .

Ma Tao, He Ruirong, Wang Chuan. Effects of phytoestrogen genistein on delayed afterdepolarization and triggered activity induced by ouabain in guinea pig papillary muscles. Acta Physiol Sin 2002; 54 (5): (in Chinese with English abstract).