过刊浏览

电刺激下丘脑外侧区对大鼠胃缺血-再灌注损伤的影响

周秀萍, 张建福, 阎长栋, 张咏梅

徐州医学院生理学教研室.江苏,徐州 221002

摘要

以0.2、0.4、0.6mA的电流强度刺激下丘脑外侧区(LHA),胃缺血-再灌注损伤(GI-RI)均显著加重,且有强度-效应依赖关系;LHA内注射L-谷氨酸(L-Glu)后,对GI-RI的效应与电刺激相似;电损毁双侧LHA,GI-RI面积较电刺激组明显减小;损毁双侧背侧迷走复合体(DVC)或切断膈下迷走神经均能取消电刺激LHA加重GI-RI的作用;电刺激LHA使缺血-再灌注(I-R)的胃粘膜丙二醛(MDA)含量升高、超氧化物歧化酶(SOD)活性降低;电刺激LHA使I-R的胃液量和总酸排出量增多;而酸度、胃蛋白酶活性和胃壁结合粘液量等无明显改变。

关键词： 下丘脑外侧区; 胃; 缺血; 再灌注损伤; 孤束核; 迷走运动核; 迷走神经; 丙二醛; 超氧化物歧化酶; 大鼠

Effects of electrical stimulation of lateral hypothalamic area on gastric ischemia-reperfusion injury in rats

Zhou Xiuping, Zhang Jianfu, Yan Changdong, Zhang Yongmei

Department of Physiology,Xuzhou Medical College.Xuzhou 221002,Jiangsu

Abstract

Electrical stimulation of LHA could aggravate GI-RI in an intensity-dependent manner by using 0.2, 0.4 or 0.6 mA current respectively. Microinjection of L-glutamic acid into LHA resulted in a similar effect to that of electrical stimulation of LHA on GI-RI. After electrolytic lesion of bilateral LHA, the are of gastric mucosal injury induced by gastric ischemia-reperfusion (GI-R) was smaller than that by electrical stimulation of LHA plus GI-R. Dorsal vagal complex (DVC) lesion or vagotomy could eliminate the effect of electrical stimulation of LHA on GI-RI. Electrical stimulation of LHA increased the content of malondialdehyde (MDA) but decreased the activity of superoxide dismutase (SOD) of ischemia-reperfusion (I-R) gastric mucosa. Electrical stimulation of LHA plus gastric I-R increased gastric juice volume and total acid output, but there were no significant changes in acidity, pepsin activity and gastric barrier mucus.

Key words: Hypothalamic area;Lateral;Gastric;Ischemia;Reperfusion injury;Solitary nucleus;Vagal motor nucleus;Vagus nerve;Malondialdehyde;Superoxide dismutase

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

周秀萍, 张建福, 阎长栋, 张咏梅. 电刺激下丘脑外侧区对大鼠胃缺血-再灌注损伤的影响[J]. 生理学报 2002; 54 (5): .

Zhou Xiuping, Zhang Jianfu, Yan Changdong, Zhang Yongmei. Effects of electrical stimulation of lateral hypothalamic area on gastric ischemia-reperfusion injury in rats. Acta Physiol Sin 2002; 54 (5): (in Chinese with English abstract).