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兔左室壁三层心肌细胞的分离及动作电位、钙和钾电流分布的异质性

李泱, 马杰, 肖建民, 刘念, 牛惠燕, 陆再英

华中科技大学同济医学院同济医院心内科.湖北,武汉 430030

摘要

探讨兔左室壁三层心肌单个细胞的分离方法以及电生理特征。实验以胶原酶按二步消化法分离兔心肌细胞,其中用剃须刀分离左室游离壁内、中、外三层心肌。采用全细胞膜片钳记录AP和离子电流。结果显示:(1)中层细胞上的动作电位时程明显长于内膜下心肌和外膜下心肌,且存在显著的1相切迹和2相驼峰;(2)中层细胞的I_(Ca,L)和I_(to)较内、外膜下的大,I_(K,s)相反。可见三层心肌细胞上多种电流存在显著差异。

关键词： 异质性; 左室壁三层心肌细胞; 动作电位; 离子电流; 兔

Heterogeneity of action potential and ion currents in the left ventricular myocytes of the rabbit

Li Yang, Ma Jie, Xiao Jianmin, Liu Nian, Niu Huiyan, Lu Zaiying

Department of Cardiology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology.Wuhan 430030,Hubei

Abstract

The results showed that the action potential and the ion currents in the three layers were significantly different. M cells had a more pronounced spike-and-dome configuration, with a significantly larger phase 1 magnitude and plateau voltage. Action potential duration (APD) in M cells was longer than that in Epi or Endo cells I_(Ca,L) and I_(to) in M cells were higher than those ofo Epi and Endo. On the contrary, I_(K,s) in M cells was the minimum compared with those in the three LV walls. The differences in ion currents may well explain the heterogeneity of action potentials in M layers of the rabbit heart.

Key words: Heterogeneity;Free wall of left ventricular myocytes;Action potentials;Ion channels;Rabbit

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引用本文：

李泱, 马杰, 肖建民, 刘念, 牛惠燕, 陆再英. 兔左室壁三层心肌细胞的分离及动作电位、钙和钾电流分布的异质性[J]. 生理学报 2002; 54 (5): .

Li Yang, Ma Jie, Xiao Jianmin, Liu Nian, Niu Huiyan, Lu Zaiying. Heterogeneity of action potential and ion currents in the left ventricular myocytes of the rabbit. Acta Physiol Sin 2002; 54 (5): (in Chinese with English abstract).